This protocol details how to perform 10 day chronic social defeat stress in early adolescent male mice to produce long-lasting social avoidance in adulthood in more than 60% of mice.
Mayeaux, M. R., Newman, E. L., Ressler, K. J., Hisey, E. E. Chronic Social Defeat Stress in Early Adolescent Male Mice. J. Vis. Exp. (215), e67113, doi:10.3791/67113 (2025).