Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Luis C. Vesga; Jonny E. Duque Luna; Stelia C. Mendez-Sanchez

doi:10.3791/67146

Journal

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Biochemistry

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Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

JoVE Journal
Biochemistry
Author Produced

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JoVE Journal Biochemistry

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

533 Views

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08:03 min

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March 07, 2025

DOI:

10.3791/67146-v

DOI:

10.3791/67146-v

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08:03 min

March 07, 2025

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524 Views

Luis C. Vesga^1,2, Jonny E. Duque Luna², Stelia C. Mendez-Sanchez¹

¹Grupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Química,Universidad Industrial de Santander ²Centro de Investigaciones en Enfermedades Tropicales – Cintrop. Escuela de Medicina, Departamento de Ciencias Básicas, Universidad Industrial de Santander,Parque Tecnológico y de Investigaciones Guatiguará Km 2 El Refugio Piedecuesta

Summary

This study introduces a methodology for studying xenobiotic effects on mitochondrial function via respirometric and enzymatic assays. It examines electron transport chain modulation in various species, offering insights for targeted biopesticide development against insect vector-borne diseases.