This study presents a method for correlative imaging of drugs in soft tissues using nonlinear optical spectroscopy and mass spectrometry imaging. Combining high-resolution skin imaging with sensitive drug detection offers a valuable approach to study drug distribution in the skin, critical for the development of superior topical products.
Tyagi, V., Dexter, A., Vorng, J., Tsikritsis, D., Gilmore, I. S., Guy, R. H., Boncheva Bettex, M., Belsey, N. A. Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section. J. Vis. Exp. (220), e67383, doi:10.3791/67383 (2025).