Name: Laparoscopic S7 Hepatectomy with Positive Fluorescence Staining (Video) | JoVE Journal
Uploaded: 2025-05-09T13:00:00.000+00:00
Duration: 7 min 43 s
Description: Learn laparoscopic S7 hepatectomy using fluorescence staining and ultrasound-guided portal vein puncture. This technique enables precise anatomical liver resection with improved surgical outcomes.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

The authors have nothing to disclose.

This protocol followed the guidelines of the Human Research Ethics Committee of the Foshan Fosun Chancheng Hospital. Written informed consent was obtained from patient for participation in this study.
1. Preoperative preparation
<ol>
	<li>Patient preparation: The patient was placed in a supine position, with the head elevated and feet lowered and tilted to the left by approximately 30°. General anesthesia was administered, including tracheal intubation. Abdominal disinfection and draping of the surgical area were performed.</li>
	<li>Trocar layout: A 1.2 cm trocar (observation hole) was inserted after cutting the skin horizontally 1 cm to the right of the navel with a surgical knife. Then, a 1.0 cm trocar was inserted at the intersection of the midclavicular line, 5 cm below the right costal margin, a 0.5 cm trocar was inserted below the right costal margin and the axilla, a 1.2 cm trocar was inserted below the xiphoid process, and a 0.5 cm trocar 3 cm to the left of the midpoint of the line connecting the umbilicus and the xiphoid process. The surgeon stands on the right, and the assistant on the left of the patient. The camera was placed in the observation hole.</li>
	<li>Abdominal exploration: Intraoperative ultrasound scans were performed along the portal and hepatic veins to determine the relationship between the tumor and the duct, confirmed by three-dimensional reconstruction. Laparoscopic exploration of the liver and abdominal cavity revealed no other lesions or metastases. Ultrasound localization of the anterior portal (AP) and PP revealed that the PP was type B. 
	NOTE: Intraoperative ultrasound evaluates the tumor size, location, intrahepatic metastasis, and its relationship with the surrounding blood vessels.</li>
</ol>
2. Surgical procedure
<ol>
	<li>Separation of the perihepatic ligament: An ultrasonic knife was used to cut off the round and falciform ligaments of the liver. The second hepatic portal was dissected, exposing the root of the right hepatic vein. The right coronary ligament and the right triangular ligament were cut. A stitch was used to ligate the three short hepatic veins dorsally to the right side of the inferior vena cava to completely free the right liver.</li>
	<li>Occlusion band: An ultrasonic knife was used to free the adhesions around the gallbladder to expose the foramen of the Venturi. Stomach gastric forceps were used along the foramen of Venturi and an occlusion band was placed in the first hepatic portal.</li>
	<li>Puncture and resection
	<ol>
		<li>PPc was visible through the right main operation port on the diaphragmatic surface, and PPd was visible on the visceral surface. The PPc and PPd were punctured using intraoperative ultrasonography guidance with a probe and a puncture hole.</li>
		<li>Diaphragmatic surface puncture (Figure 3 and Figure 4): The probe was inserted into the main operative hole under the right costal margin. The PPC was visible on the diaphragmatic surface, and the long diameter of the PPC was exposed. The puncture point from the root of the PPC was selected. A 21G percutaneous transhepatic cholangial (PTC) needle was used to prick the bile duct. The one face, three points, and four horizontal fingers method was used.
		<ol>
			<li>On one side, the midpoint of the left and right adjustment rods was used for intraoperative ultrasound as the aiming point, and the probe rod was used as the vertical plane for in-plane puncture. The three points selected were the skin puncture point, the intraoperative probe puncture hole, and the target liver pedicle puncture point. The length of the four horizontal fingers was used to measure the skin puncture point approximately at the intersection of the vertical plane of the probe rod and the skin in front of the trocar.</li>
		</ol>
		</li>
		<li>The PTC needle was held so the bevel faces the ventral distal side. The needle core was removed, and 3 mL of 0.025 mg/mL indocyanine green was slowly injected. The diaphragmatic surface was visualized using fluorescence imaging.</li>
		<li>Visceral surface puncture (Figure 5 and Figure 6): The puncture hole location was selected under the xiphoid process for probe insertion. PPd was selected as the puncture point, and 3 mL of 0.025 mg/mL indocyanine green dilution was slowly injected.</li>
		<li>At this time, the visceral surface of the liver S7 segment was fluorescently imaged and used to determine the resection margin. An elastic traction rope was used to pull the liver S7 segment from the lower edge of the liver S6 segment to the left lower abdomen.</li>
		<li>The liver tissue was cut from the caudal to the cephalic side and along the boundary between the fluorescent and non-fluorescent segments, along the interterritory hepatic vein (IVH) between liver segments S6 and S7 and the right hepatic vein (Figure 7).</li>
		<li>Along the right edge of the right hepatic vein, the liver S7 reflux vein was ligated using ligating clips and disconnected from the two branch liver pedicles of liver section S7. An ultrasonic scalpel and bipolar electrocoagulation were used to cut the liver tissue using fluorescent imaging boundaries under anesthesia-assisted low central venous pressure.</li>
		<li>Hemostasis of residual liver: The residual liver was carefully checked, and the bleeding points were closed one by one using bipolar electrocoagulation. Coated Vicryl antibacterial suture was used to stitch close the incision.</li>
		<li>For postoperative pain, intravenous analgesics were administered. During postoperative observation, changes in liver function and bilirubin levels were measured.</li>
	</ol>
	</li>
</ol>
<img alt="Minimal invasive surgery tools in use; includes ultrasound needle guidance; surgical setup." class="xfigimg" src="/files/ftp_upload/67493/67493fig03.jpg" data-altupdatedat="1788304762000" data-alt="Figure 3"> 
Figure 3: Diaphragmatic surface puncture. (A) Intraoperative ultrasound enters along the main operating hole. (B) Laparoscopic PTC puncture needle is used to puncture along the intraoperative ultrasound fixation hole. (C) PTC puncture needle (white arrow) puncture PPc portal vein image under ultrasound guidance. <a href="https://www.jove.com/files/ftp_upload/67493/67493fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Fluorescence-guided laparoscopic surgery method; surgical instruments, tissue dye visualization." class="xfigimg" src="/files/ftp_upload/67493/67493fig04.jpg" data-altupdatedat="1788304762000" data-alt="Figure 4"> 
Figure 4: Diaphragmatic surface puncture positive staining. PPc portal vein positive staining picture and mark the stained edges with an ultrasonic knife. The green area shows the portal territory to which PPc belongs. <a href="https://www.jove.com/files/ftp_upload/67493/67493fig04large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Laparoscopic needle insertion; includes surgical procedure, needle ultrasound, and anatomical imagery." class="xfigimg" src="/files/ftp_upload/67493/67493fig05.jpg" data-altupdatedat="1788304762000" data-alt="Figure 5"> 
Figure 5: Visceral surface puncture. (A) Intraoperative ultrasound enters along the assistant operation hole. (B) Laparoscopic PTC puncture needle is used to puncture along the intraoperative ultrasound fixation hole. (C) PTC puncture needle (white arrow) puncture PPd portal vein image under ultrasound guidance. <a href="https://www.jove.com/files/ftp_upload/67493/67493fig05large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Fluorescence-guided surgery process; indocyanine green highlights tissue during endoscopy procedure." class="xfigimg" src="/files/ftp_upload/67493/67493fig06.jpg" data-altupdatedat="1788304762000" data-alt="Figure 6"> 
Figure 6: Visceral surface puncture positive staining. PPd portal vein positive staining picture and mark the stained edges with an ultrasonic knife. The green area shows the portal territory to which PPd belongs. <a href="https://www.jove.com/files/ftp_upload/67493/67493fig06large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Liver anatomy labeled diagram showing portal vein branches and hepatic veins for medical education." class="xfigimg" src="/files/ftp_upload/67493/67493fig07.jpg" data-altupdatedat="1788304762000" data-alt="Figure 7"> 
Figure 7: IHV and the right hepatic vein. The purple area shows the right hepatic vein; the green area shows segments of 7 branches of the hepatic vein; the yellow area is a cross-section of the PPC and PPD; the red area is the right posterior hepatic pedicle. Abbreviations: v7-1= The first one of the S7 segment branches of the hepatic vein; v7-2= The second one of S7 segment branches of the hepatic vein; RHV = right hepatic vein; PPc: C branch of the portal vein in the right posterior lobe of the liver; PPd: D branch of the portal vein in the right posterior lobe of the liver. <a href="https://www.jove.com/files/ftp_upload/67493/67493fig07large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

<ol><li>Han, B. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cancer+incidence+and+mortality+in+China%2C+2022%5BTitle%5D)+AND+%22J+Natl+Cancer+Cent%22%5BJournal%5D)">Cancer incidence and mortality in China, 2022.</a> J Natl Cancer Cent. 4 (1), 47-53 (2024).</li>
<li>Ishizawa, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Laparoscopic+segmentectomy+of+the+liver%3A+from+segment+I+to+VIII%5BTitle%5D)+AND+%22Ann+Surg%22%5BJournal%5D)">Laparoscopic segmentectomy of the liver: from segment I to VIII.</a> Ann Surg. 256 (6), 959-964 (2012).</li>
<li>Makuuchi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ultrasonically+guided+subsegmentectomy%5BTitle%5D)+AND+%22Surg+Gynecol+Obstet%22%5BJournal%5D)">Ultrasonically guided subsegmentectomy.</a> Surg Gynecol Obstet. 161 (4), 346-350 (1985).</li>
<li>Takamoto, T., Makuuchi, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Precision+surgery+for+primary+liver+cancer%5BTitle%5D)+AND+%22Cancer+Biol+Med%22%5BJournal%5D)">Precision surgery for primary liver cancer.</a> Cancer Biol Med. 16 (3), 475-485 (2019).</li>
<li>Cho, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Relation+between+hepatic+and+portal+veins+in+the+right+paramedian+sector%3A+proposal+for+anatomical+reclassification+of+the+liver%5BTitle%5D)+AND+%22World+J+Surg%22%5BJournal%5D)">Relation between hepatic and portal veins in the right paramedian sector: proposal for anatomical reclassification of the liver.</a> World J Surg. 28, 8-12 (2004).</li>
<li>Shindoh, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Complete+removal+of+the+tumor-bearing+portal+territory+decreases+local+tumor+recurrence+and+improves+disease-specific+survival+of+patients+with+hepatocellular+carcinoma%5BTitle%5D)+AND+%22J+Hepatol%22%5BJournal%5D)">Complete removal of the tumor-bearing portal territory decreases local tumor recurrence and improves disease-specific survival of patients with hepatocellular carcinoma.</a> J Hepatol. 64 (3), 594-600 (2016).</li>
<li>Shindoh, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+intersegmental+plane+of+the+liver+is+not+always+flat+-+tricks+for+anatomical+liver+resection%5BTitle%5D)+AND+%22Ann+Surg%22%5BJournal%5D)">The intersegmental plane of the liver is not always flat - tricks for anatomical liver resection.</a> Ann Surg. 251 (5), 917-922 (2010).</li>
<li>Ciria, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+snapshot+of+the+2020+conception+of+anatomic+liver+resections+and+their+applicability+on+minimally+invasive+liver+surgery.+A+preparatory+survey+for+the+expert+consensus+meeting+on+precision+anatomy+for+minimally+invasive+HBP+surgery%5BTitle%5D)+AND+%22J+Hepatobiliary+Pancreat+Sci%22%5BJournal%5D)">A snapshot of the 2020 conception of anatomic liver resections and their applicability on minimally invasive liver surgery. A preparatory survey for the expert consensus meeting on precision anatomy for minimally invasive HBP surgery.</a> J Hepatobiliary Pancreat Sci. 29 (1), 41-50 (2022).</li>
<li>Zheng, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Laparoscopic+anatomical+portal+territory+hepatectomy+with+cirrhosis+by+takasaki%27s+approach+and+indocyanine+green+fluorescence+navigation+%28with+Video%29%5BTitle%5D)+AND+%22Ann+Surg+Oncol%22%5BJournal%5D)">Laparoscopic anatomical portal territory hepatectomy with cirrhosis by takasaki's approach and indocyanine green fluorescence navigation (with Video).</a> Ann Surg Oncol. 27 (13), 5179-5180 (2020).</li>
<li>Kawaguchi, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Difficulty+of+laparoscopic+liver+resection%3A+proposal+for+a+new+classification%5BTitle%5D)+AND+%22Ann+Surg%22%5BJournal%5D)">Difficulty of laparoscopic liver resection: proposal for a new classification.</a> Ann Surg. 267 (1), 13-17 (2018).</li>
<li>Liang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Laparoscopic+anatomical+portal+territory+hepatectomy+using+Glissonean+pedicle+approach+%28Takasaki+approach%29+with+indocyanine+green+fluorescence+negative+staining%3A+how+I+do+it%5BTitle%5D)+AND+%22HPB%22%5BJournal%5D)">Laparoscopic anatomical portal territory hepatectomy using Glissonean pedicle approach (Takasaki approach) with indocyanine green fluorescence negative staining: how I do it.</a> HPB. 23 (9), 1392-1399 (2021).</li>
<li>Ferrero, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Laparoscopic+right+posterior+anatomic+liver+resections+with+Glissonean+pedicle+-first+and+venous+craniocaudal+approach%5BTitle%5D)+AND+%22Surg+Endosc%22%5BJournal%5D)">Laparoscopic right posterior anatomic liver resections with Glissonean pedicle -first and venous craniocaudal approach.</a> Surg Endosc. 35 (1), 449-455 (2021).</li>
<li>Morise, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Laparoscopic+liver+resection+for+posterosuperior+tumors+using+caudal+approach+and+postural+changes%3A+a+new+technical+approach%5BTitle%5D)+AND+%22World+J+Gastroenterol%22%5BJournal%5D)">Laparoscopic liver resection for posterosuperior tumors using caudal approach and postural changes: a new technical approach.</a> World J Gastroenterol. 2016 (47), 10267-10274 (2016).</li>
<li>Okuda, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intrahepatic+Glissonean+pedicle+approach+to+segment+7+from+the+dorsal+side+during+laparoscopic+anatomic+hepatectomy+of+the+cranial+part+of+the+right+liver%5BTitle%5D)+AND+%22J+Am+Coll+Surg%22%5BJournal%5D)">Intrahepatic Glissonean pedicle approach to segment 7 from the dorsal side during laparoscopic anatomic hepatectomy of the cranial part of the right liver.</a> J Am Coll Surg. 226 (2), e1-e6 (2018).</li>
<li>Cao, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Totally+laparoscopic+anatomic+S7+segmentectomy+using+in+situ+split+along+the+right+intersectoral+and+intersegmental+planes%5BTitle%5D)+AND+%22Surg+Endosc%22%5BJournal%5D)">Totally laparoscopic anatomic S7 segmentectomy using in situ split along the right intersectoral and intersegmental planes.</a> Surg Endosc. 35 (1), 174-181 (2021).</li>
<li>Wang, X., Tong, H., Li, J., Wang, H. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Indocyanine+green+fluorescence-guided+laparoscopic+anatomical+segmentectomy+of+liver+segment+6%3A+Surgical+strategy+and+technical+details%5BTitle%5D)+AND+%22Ann+Surg+Oncol%22%5BJournal%5D)">Indocyanine green fluorescence-guided laparoscopic anatomical segmentectomy of liver segment 6: Surgical strategy and technical details.</a> Ann Surg Oncol. 31 (10), 6546-6550 (2024).</li>
</ol>

Currently, liver surgery is entering an era of minimally invasive surgery. There have been reports of anatomical resection of various liver segments in patients with HCC, and the safety of this operation has been verified2. Its efficacy in oncology has attracted increasing attention, and the anatomical hepatectomy approach proposed by Shindoh et al. involves complete resection based on tumor-related territory staining6. Limited by previous techniques and equipment, only Cou...

Primary liver cancer is currently the fourth most common malignant tumor and the second leading cause of cancer-related death in China, posing a serious threat to the lives and health of people globally1. For hepatocellular carcinoma (HCC), surgical resection has long been the primary treatment option. With the advancements in minimally invasive technology, there has been a growing number of reports on laparoscopic anatomical liver resection for treating HCC. For laparoscopic hepatectomy located in various liver segments, including specialized segments (I, IVb, VII, and VIII), relevant studies have shown that this method is safe and effective2.
The concept of anatomical liver resection was first proposed by Makuuchi et al. in 19853,4. The correct procedure is to segment according to the portal vein territory staining and perform complete resection of the portal vein territory staining to which the tumor belongs5. As HCC mainly spreads along the portal vein, in theory, this approach can provide better oncological efficacy and achieve true anatomical liver resection for tumors in different locations6. However, in the past, due to limitations in technology and equipment, this treatment was uncommon. Most centers performed anatomical liver resection based on the Couinaud liver segmentation method. When the tumor spans multiple liver segments, performing anatomical liver resection can result in excessive removal of healthy liver tissue, thereby increasing surgical risk and postoperative complications. Additionally, potential micrometastatic lesions may persist due to incomplete resection of the tumor portal vein territory7,8.
With the advancements in technology and equipment, we can define the tumor portal vein territory based on preoperative three-dimensional reconstruction. This helps clinicians determine the resection range, perform ultrasound-guided puncture and staining of the portal vein territory staining branches to which the tumor belongs during surgery, and determine the liver section plane using indocyanine green fluorescence imaging to achieve more accurate anatomical liver resection9. However, for laparoscopic anatomical liver resection of liver segment S7, the operation is more challenging because the liver pedicle is deeply hidden in the liver parenchyma and localized proximally to the dorsal and cephalad sides, thus resulting in a longer operation time and greater trauma10. The positive staining method involves direct puncture of the corresponding portal vein under ultrasound guidance (typically used in liver segments S7 and S8) so that the target liver segment is directly stained. This helps the surgeon avoid cutting an excessive amount of liver parenchyma, thereby maximizing the protection of functional liver volume11. However, the positive staining method requires a specific intraoperative ultrasound foundation, the correct identification of intrahepatic ducts, and the appropriate use of intraoperative portal vein puncture techniques. It also places a high demand on the surgeon and is associated with a prerequisite learning curve.
In the patient described here, the tumor was located in segment S7 of the liver. The preoperative three-dimensional reconstruction revealed two portal vein branches. Because the S7 trunk is short and close to the root of the segment S6 portal vein, it was punctured along the diaphragmatic and visceral surfaces of the liver. Indocyanine green was injected to stain the target liver segment, and the liver was resected following the fluorescent signal that guided the procedure and ensured smooth operation.
The aim of the liver S7 segment resection method demonstrated here is to further promote the concept of portal territory staining-guided anatomic liver resection and highlight the advantages of S7 segment-positive staining resection. This procedure minimizes the volume of healthy liver tissue removed during tumor resection while maximizing tumor removal efficiency.
CASE PRESENTATION:
A 30-year-old male was admitted to Foshan Fosun Chancheng Hospital on 2023-02-02. The patient was found to have a space-occupying lesion in his liver at another hospital 1 month prior, without discomfort. He otherwise had a history of good health.
Diagnosis, Assessment, and Plan: 
Diagnosis: Hepatocellular carcinoma.
Assessment: ALT (alanine aminotransferase): 123 U/L, AST (aspartate aminotransferase): 34 U/L, hemoglobin: 141 g/L, platelet count: 125 x 109 cells/L, albumin: 40.5 g/L, total bilirubin: 10.1 µmol/L, creatinine: 67 µmol/L, prothrombin time (PT): 14.1 s, hepatitis B surface antigen positive, HBV DNA(Hepatitis B virus DNA): 3.51 x 106 IU/L, abnormal prothrombin (PIVKA-II): 21 mAU/mL, AFP (alpha-fetoprotein): 56.29 µg/L, CA199 (carbohydrate antigen199): &lt;0.8 U/mL, CEA (carcinoembryonic antigen): 4.65 U/mL, cholinesterase: 7128 U/L, Child-Pugh grade A. Enhanced CT (computerized tomography scan) and enhanced (gadoxetate disodium) MRI (magnetic resonance imaging) of the upper abdomen: 1 cm mass in the liver S7 segment, three-dimensional reconstruction territory analysis (see Figure 1). The remaining liver volume was 78.8%.
<img alt="3D anatomical diagram; liver vasculature; focus on veins, labeled branches; medical study aid." class="xfigimg" src="/files/ftp_upload/67493/67493fig01.jpg" data-altupdatedat="1788304762000" data-alt="Figure 1"> 
Figure 1: Three-dimensional reconstruction analysis. The location of the tumor, three-dimensional reconstruction of the tumor-related portal and hepatic veins, and important blood vessels near the tumor. Abbreviations: v7 = segments of 7 branches of a hepatic vein; PPC = posterior portal vein C; PPD = posterior portal vein D; IHV = inter territory hepatic vein; RHV = right hepatic vein. <a href="https://www.jove.com/files/ftp_upload/67493/67493fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
Plan: Laparoscopic S7 hepatectomy with fluorescence-positive staining was planned. Step 1: CT and three-dimensional reconstruction were used for territory analysis. The tumor was located in the posterior portal vein C (PPc) and posterior portal vein D (PPd) territories of the S7 segment of the liver (referring to the right posterior portal vein classification of Japanese scholars4; refer Figure 2). Step 2: Intraoperative ultrasound showed that the portal vein to which the tumor belonged had two vascular branches. Step 3: Target liver pedicles PPc and PPd were severed, and the veins between liver regions S6 and S7 and the right hepatic vein were fully exposed under fluorescence guidance. Step 4: Tumor resection guided by fluorescent staining.
<img alt="CT scan comparison showing pancreatic duct changes and tumor presence, with labeled markers." class="xfigimg" src="/files/ftp_upload/67493/67493fig02.jpg" data-altupdatedat="1788304762000" data-alt="Figure 2"> 
Figure 2. Preoperative CT scan. (A) CT section of tumor-associated portal vein of PPd (red arrow). (B) CT section of tumor-associated portal vein of PPc. <a href="https://www.jove.com/files/ftp_upload/67493/67493fig02large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

<table><tbody><tr><td>Coated Vicryl Plus Antibacterial Suture</td><td>Ethicon, Inc.</td><td>3650118</td><td>The product is suitable for the placement and/or ligation of soft tissues</td></tr><tr><td>Color Doppler ultrasound diagnostic scanner</td><td>BK Medical</td><td>20153251933</td><td>intraoperative ultrasound</td></tr><tr><td>Disposable laparoscopic puncture device and puncture sheath</td><td>Jiangsu Fenghe Medical Equipment Co., Ltd</td><td>20182021588</td><td>Used for laparoscopic examination and surgical procedures, to puncture the abdominal wall tissue of the human body and establish a working channel for abdominal surgery</td></tr><tr><td>Four way curved electron convex array laparoscopic intraoperative probe</td><td>BK Medical</td><td>20153251933</td><td>Used for intraoperative examination and interventional treatment in various laparoscopic surgeries</td></tr><tr><td>HAKKO SONOGUIDE PTC NEEDLE</td><td>Baguang Trading (Shanghai) Co., Ltd</td><td>20172146872</td><td>Percutaneous liver bile duct puncture needle</td></tr><tr><td>Indocyanine Green for Injection</td><td>DANDONG YICHUANG PHARMACEUTICAL</td><td>ICP-09018669-1</td><td>Assessment of liver reserve function and liver imaging</td></tr><tr><td>WECK Hem-o-lok</td><td>Teleflex Medical</td><td>20143466018</td><td>Ligation of blood vessels or tissues</td></tr></tbody></table>

laparoscopic s7 hepatectomy with positive fluorescence staining

Laparoscopic S7 Hepatectomy with Positive Fluorescence Staining

Laparoscopic liver resection for tumors located in the S7 segment of the liver typically adopts a traditional surgical approach. The main goal of this procedure is to accurately dissect the liver pedicle of the S7 segment. Dissecting the hepatic pedicle of the S7 segment along the hepatic hilum requires a relatively long path within the liver, which increases the risk of losing orientation and potentially injuring the adjacent hepatic pedicle of the S5 and S6 segments, thereby compromising the liver resection plane. A positive staining method was used to directly puncture the corresponding portal vein under ultrasound guidance (commonly for S7 and S8 segments) to specifically color the target liver segment, thereby avoiding extensive resection of the liver parenchyma, and reducing damage to the surrounding healthy liver tissue. However, the positive staining method requires a specific foundation in intraoperative procedures, which can be challenging for surgeons and has a certain learning curve. Currently, technologies such as three-dimensional reconstruction territory analysis, intraoperative ultrasound, and indocyanine green fluorescence imaging are popular and commonly used in laparoscopic liver resection. In this protocol, under laparoscopic ultrasound guidance, the tumor basin was punctured through the visceral and diaphragmatic surfaces of the liver to stain segment S7. Laparoscopic resection of segment S7 within the portal territory anatomic liver was successfully performed, further confirming the feasibility and advantages of positive fluorescence staining in laparoscopic liver resection at this stage.

In this case report, laparoscopic portal territory staining-guided anatomical liver resection of the S7 segment was successfully performed, with positive staining along the diaphragmatic and visceral surfaces (Figure 6 and Figure 7). Resection of a single 1 cm tumor was done. The operative time was 210 min, and intraoperative blood loss was 100 mL. The postoperative hospitalization duration was 7 days, and the patient is still under continuous f...

The protocol demonstrates that, under laparoscopic ultrasound guidance, the S7 segment of the liver can be successfully stained by puncturing the visceral and diaphragmatic branches of the tumor, facilitating an anatomical hepatectomy of the S7 segment.

Laparoscopic liver resection for tumors located in the S7 segment of the liver typically adopts a traditional surgical approach. The main goal of this ...

laparoscopic-s7-hepatectomy-with-positive-fluorescence-staining

Research

JoVE Journal

Medicine

High-grade hemostatic devices are essential for laparoscopic hepatectomy. However, these devices are not generalized in basic medical organizations. Therefore, a suite of simple and easy hemostatic devices is shown in this article, which can make the laparoscopic hepatectomy easier to perform.

Application of Hemostatic Devices in Laparoscopic Hepatectomy

Numerous studies have demonstrated the advantages of anatomic resection. Nonetheless, whether anatomic resection can increase R0 resection rates remains controversial. Consequently, the present study describes an innovative procedure involving the en bloc concept combined with anatomic resection in laparoscopic hepatectomy, which can reduce postoperative recurrence and metastasis.

Application of the En Bloc Concept Combined with Anatomic Resection in Laparoscopic Hepatectomy

The present protocol describes fluorescent negative staining in laparoscopic central hepatectomy. This technique can make hepatectomy more accurate and precise.

Fluorescent Laparoscopic Central Hepatectomy for Liver Cancer Using Indocyanine Green Negative Staining

Here we present a step-by-step protocol for conducting laparoscopic anterior right hepatectomy and compare its clinical effects and postoperative outcomes with those of conventional hepatectomies. Analysis of the data of 82 patients with hepatocellular carcinoma revealed that laparoscopic anterior right hepatectomy had better clinical outcomes and survival rates than the conventional hepatectomy.

Laparoscopic Anterior Right Hepatectomy: A Single-Center Experience

This protocol involves a laparoscopic anatomical resection of liver segment VII with liver parenchymal transection following a priority approach. This technique is unaffected by anatomical variations.

Laparoscopic Anatomical Liver Segment VII Resection with Liver Parenchymal Transection Following a Priority Approach

Intrahepatic cholangiocarcinoma of the caudate lobe is a challenge for many surgeons due to its unique location. Here, we present a protocol to show the step-by-step details of laparoscopic left hemihepatectomy combined with caudate lobe resection for cholangiocarcinoma.

Laparoscopic Left Hemihepatectomy Combined with Caudate Lobe Resection

Here, we present a protocol for laparoscopic anatomical hepatectomy using Takasaki's approach and indocyanine green fluorescence navigation in S4/5/7/8 resection.

Laparoscopic Anatomical Hepatectomy Using Takasaki's Approach and Indocyanine Green Fluorescence Navigation

We present a laparoscopic technique for anatomic S7+S8d resection with right hepatic vein transection, preserving the inferior right hepatic vein and segment S6. Relying on meticulous anatomical landmarks without intraoperative ultrasound, this approach offers a precise and parenchyma-sparing option for dorsally located right-liver tumors, particularly in settings with limited advanced navigation resources.

Laparoscopic Anatomic S7+S8d Resection Preserving Inferior Right Hepatic Vein and S6 with Right Hepatic Vein Transection

Here, a protocol involving re-arterialized rat partial liver transplantation is presented. Specifically, 70% liver was resected in vivo by using an updated technique of vessel-oriented hepatectomy. The hepatic artery was reconstructed in an end-to-side manner. The cuff technique was modified to shorten the anastomosis time of the infrahepatic vena cava.

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

Robotic liver surgery has gained more acceptance as a feasible, safe, and effective procedure for the treatment of both benign and malignant indications. However, robotic left hepatectomy is still technically demanding. We describe our surgical technique of a robotic left hepatectomy using indocyanine green fluorescence imaging for a large biliary cyst.

Robotic Left Hepatectomy using Indocyanine Green Fluorescence Imaging for an Intrahepatic Complex Biliary Cyst

Learn laparoscopic S7 hepatectomy using fluorescence staining and ultrasound-guided portal vein puncture. This technique enables precise anatomical liver resection with improved surgical outcomes.

Laparoscopic S7 Hepatectomy with Positive Fluorescence Staining

Explore More Videos