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February 21, 2025
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This protocol outlines a detailed procedure for generating transient chimeric antigen receptor (CAR) T cells using non-integrating mRNA for cancer immunotherapy and provides reliable methods for evaluating CAR-T cells and their cytotoxic function.
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Cite this Article
Hu, L., Berahovich, R., Huang, Y., Zhang, S., Sun, J., Liu, X., Zhou, H., Xu, S., Li, H., Golubovskaya, V., Wu, L. A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy. J. Vis. Exp. (216), e67548, doi:10.3791/67548 (2025).
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