Detection of <em>Helicobacter pylori</em> Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Jin-Xin Lai; Hui-Min Chen; Cheng-Yuan He; Yu-Ting Si; Luan Luan; Ge Huang; You-Wei Zheng; Yu-Wei Di; Xin-Qiang Zhang; Ya-Nan Yao; Zheng-Kang Li; Liang Wang

doi:10.3791/67710

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitat...

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

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04:56 min

May 16, 2025

DOI: 10.3791/67710-v

Jin-Xin Lai*¹, Hui-Min Chen*¹, Cheng-Yuan He*¹, Yu-Ting Si², Luan Luan², Ge Huang¹, You-Wei Zheng¹, Yu-Wei Di¹, Xin-Qiang Zhang¹, Ya-Nan Yao¹, Zheng-Kang Li¹, Liang Wang¹

¹Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences),Southern Medical University, ²Medical Technology School,Xuzhou Medical University

The protocol employs a non-invasive stool sampling combined with a quantitative polymerase chain reaction to offer a convenient and rapid diagnostic method for Helicobacter pylori infection and its resistance to clarithromycin and quinolones.

This study demonstrates a qPCR-based fecal test to detect helicobacter pylori infection and antibiotic resistance, enabling personalized treatment and improving H. pylori eradication rate at the population level.

Fecal samples combined with quantity PCR enabled delayed detection of helicobacter pylori infections and antibiotic resistance without bacterial culture, reducing diagnosis done from three weeks to one day.

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