Name: Kidney Procurement in a Preclinical Large Animal Model (Video) | JoVE Journal
Uploaded: 2025-05-02T13:20:00.000+00:00
Duration: 5 min 36 s
Description: Learn kidney procurement in a preclinical swine model for transplantation research using median laparotomy and cryoprotective preservation techniques. This method helps researchers extend organ storage time and improve transplant outcomes.

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5 minutes

The authors would like to acknowledge the many veterinary technicians at Johns Hopkins University School of Medicine for their technical assistance. We would also like to express our gratitude to Drs. Jessica Izzi and Amanda Maxwell, and the numerous veterinary residents, including Drs. Mallory Brown, Jessica Plunkard, and Alexis Roach for providing our animals with excellent clinical care and veterinary oversight. Finally, we would like to acknowledge all members of the Vascularized Composite Allotransplantation (VCA) Laboratory at the Johns Hopkins School of Medicine who have assisted in any capacity with kidney procurement or other organ procurement procedures performed in our laboratory. This work was supported by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) grant R44DK136396.

All procedures described were approved by the Institutional Care and Use Committee of Johns Hopkins University, a United States Department of Agriculture (USDA) licensed, Office of Laboratory Animal Welfare (OLAW)-assured, and the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-accredited institution. Animals were maintained in accordance with the United States Department of Agriculture&#39;s Animal Welfare Act, the National Institutes of Health&#39;s Guide for the Care and Use of Laboratory Animals, and the United States Public Health Service&#39;s Policy on Humane Care and Use of Laboratory Animals.
1. Animals and housing
<ol>
	<li>Use 3-6-month-old clinically healthy 20-40 kg Yorkshire pigs (Sus scrofa domesticus), or comparable, for this protocol.</li>
	<li>House the animals in chain-link fencing runs with slatted flooring or other species-appropriate housing.</li>
</ol>
2. Preoperative procedure and anesthesia
<ol>
	<li>Fast the animal at least 12 h prior to sedation time.</li>
	<li>Administer oral simethicone (20 mg/kg) at least 12 h prior to surgery and repeat dosing at least 1 h prior to sedation to reduce bowel dilation.</li>
	<li>Administer ketamine (20-30 mg/kg) and xylazine (2-3 mg/kg) in a single syringe intramuscularly to avoid multiple injections.</li>
	<li>Transfer the pig to the preoperative staging area. Place an intravenous catheter in a marginal ear vein. Administer 3-10 mL/kg/h of 0.9% saline or Lactated Ringer&#39;s solution throughout the procedure.</li>
	<li>Once the pig is adequately sedated, place an endotracheal tube (6.0-6.5 mm cuffed endotracheal tube for 20-30 kg animal; 6.5-7.0 mm cuffed for 30-40 kg) for delivery of gas anesthesia and mechanical ventilation. If required, facilitate with a dose of propofol (0.8-1.66 mg/kg) intravenously, administered to effect.</li>
	<li>Remove the hair on and surrounding the surgical site using clippers, as well as a dirty scrub of the surgical site, before moving the animal into the operating room.</li>
	<li>Transfer the animal to the operating table. Administer cefazolin (20-22 mg/kg) intravenously 10 min prior to the start of surgery and again every 90 min intraoperatively. Administer pantoprazole (0.5-1 mg/kg) intravenously at the start of surgery.</li>
	<li>Administer a local block subcutaneously with lidocaine (up to 2 mg/kg) to facilitate added analgesia prior to incision.</li>
	<li>Aseptically prep the surgical site, alternating between chlorhexidine or betadine and 70% ethanol or saline, at least three times.</li>
	<li>Continuously monitor fluid volume, heart rate, blood pressure, pulse oximetry, capnography, electrocardiography, and rectal temperature. Record these values every 10-15 min. Use a heated underbody pad and warm-air blanket to prevent hypothermia.</li>
	<li>Prior to the initiation of surgery, confirm that the animal is within the appropriate plane of surgical anesthesia by utilizing a combination of jaw tone, palpebral reflex, and parameters listed in the previous step.</li>
</ol>
3. Kidney harvest procedure
<ol>
	<li>Prepare the animal for surgery, following the steps described in Section 2.</li>
	<li>Following sterile disinfection and draping of the surgical field, perform a median laparotomy (25-30 cm) to gain optimal access to both kidneys. Insert a standard abdominal retractor.</li>
	<li>Cover the colon and small bowel with towels soaked in warm saline. Retract the bowels to either the right side for access to the left kidney or to the left side for access to the right kidney.</li>
	<li>Open the peritoneum overlying the kidney and dissect around the kidney to free any adhesions. Dissect the ureter until 10-12 cm of length is obtained.</li>
	<li>Dissect the renal vein and artery until their origin from the inferior vena cava and the aorta, respectively.</li>
	<li>After complete renal dissection, tie the ureter distally with 2-0 silk ligature and cut proximally to the tie. Leave the proximal ureter end open to allow for urine drainage (in survival procedure, collect the urine on a gauze).</li>
	<li>Heparinize the animal with intravenous heparin (100 IU/kg) and wait for 2 min to ensure adequate heparinization of the kidney. This step should be repeated prior to the resection of each kidney.</li>
	<li>Clamp the renal artery and renal vein close to the aorta and inferior vena cava, respectively, with two Satinsky vascular clamps. Remove the kidney graft by cutting the renal artery and vein close to the clamps.</li>
	<li>Immediately cannulate the renal artery with a 3 mm blunt tip perfusion cannula. Flush the kidney with ice-cold University of Wisconsin (UW) solution or Custodiol Histidine-tryptophan-ketoglutarate (HTK) preservation solution.</li>
	<li>Remove the perfusion cannula after flushing with UW or HTK and place the kidney in a sterile organ bag filled with the same ice-cold preservation solution that was used for flushing the kidney. Place this bag within a second sterile organ bag. Subsequently, store the organ or initiate machine perfusion.</li>
	<li>Ligate the renal artery stump with a 2-0 silk ligature. Close the renal vein stump with a two-layer running suture with 6-0 polypropylene.</li>
	<li>In survival cases, flush the peritoneal cavity with sterile saline prior to closure.</li>
</ol>
4. Blood harvest procedure
<ol>
	<li>If the procedure is terminal and all other surgical interventions have been performed, proceed with harvesting blood for machine perfusion and euthanasia by exsanguination.</li>
	<li>Retract the bowels to the right side and identify the infrarenal abdominal aorta. Free any large adhesions or tissue covering the vessel.</li>
	<li>Insert the blood collection bag needle directly into the aorta. Hang the bag below the animal to facilitate filling. Once the bag is full (approximately 450 mL of blood), remove the needle from the aorta and hold pressure on the puncture site.</li>
	<li>For a second blood bag, insert the new needle 1-2 cm proximally to the previous puncture site. Repeat with multiple blood bags as needed, moving 1-2 cm proximally with each needle.</li>
	<li>After all necessary blood has been collected, induce cardiac arrest with intravenous injection of pentobarbital sodium/phenytoin sodium at a dose of at least 78 mg/kg of the pentobarbital component. If there is concern that pentobarbital will interfere with subsequent assays of the kidney, use potassium chloride (KCl) (75-100 mg/kg IV) as an alternative chemical euthanasia method if it is administered to pigs under anesthesia. Verify death by lack of heartbeat, absent corneal reflex, and a significant decrease in body temperature. 
	NOTE: The remaining organs and tissue of the euthanized pig can be used for other research or educational purposes in compliance with the 3R principles.</li>
</ol>

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</ol>

The authors have no conflicts of interest to disclose.

The porcine model for kidney transplantation has been vital to the development and optimization of machine perfusion technology. Given the anatomical, immunological, and pathophysiological similarities to human kidneys6,7,8, porcine kidneys offer a facilitated translation to clinical testing and practice.

The model of porcine kidney procurement can be utilized for a variet...

Kidney transplantation is the optimal treatment for end-stage renal disease (ESRD), providing improved quality of life and long-term outcomes as compared to dialysis1. Despite advances in organ preservation, dozens of people die each day from ESRD while on the waitlist for a kidney transplant2. Machine perfusion is a growing field that increases preservation times, enabling extended donor networks and more efficient organ allocation. This technology also allows for ex vivo organ monitoring and optimization, thereby minimizing the effects of ischemia-reperfusion injury (IRI). When compared to static cold storage (SCS), machine perfusion has been shown to significantly reduce the incidence of delayed graft function3,4. Machine perfusion also has demonstrated revitalization of marginal grafts, which would otherwise not have met the criteria for transplant5.&#160;Despite technological advancements, the most common preservation technique remains SCS on ice. Further preclinical experiments and data can help to make machine perfusion a mainstay of kidney preservation.

The porcine model for kidney transplantation is well-established and has been integral to the development of kidney preservation technology, especially machine perfusion. Unlike unilobular rodent kidneys, porcine and human kidneys are both multilobular, similar in size, and share analogous arterial, venous, and urinary anatomy6,7. Therefore, the porcine kidney facilitates a direct translation to the clinical setting, especially in terms of medical devices and drug therapies. Additionally, porcine kidneys show a similar pathophysiology of IRI as human kidneys8, making them ideal for kidney preservation studies.

In order to ensure graft quality and data reproducibility, standardized and clinically translatable surgical techniques for organ and tissue procurement should be followed. Thus, here, we describe an optimized protocol for kidney procurement in a preclinical swine model. This protocol allows the recovery of kidneys and simulates either a living (single kidney) or deceased (dual kidney) donor setting. The single kidney recovery offers the advantage of performing a subsequent autotransplantation. In the dual kidney recovery model, blood collection prior to euthanasia is possible by inserting blood bag needles directly into the aorta, thereby exsanguinating the animal and providing blood for ex vivo machine perfusion.

<table><tbody><tr><td>70% Ethanol Solution</td><td>Fisher Scientific</td><td>04-355-122</td><td></td></tr><tr><td>Adson Tissue ForceA2:D30ps, 4.75&quot;, 1 x 2 Teeth</td><td>Wexler Surgical</td><td>FL0081.1</td><td></td></tr><tr><td>Bair Hugger Animal Health Overbody Blanket</td><td>3M</td><td>53777</td><td></td></tr><tr><td>Bair Hugger Warming Unit</td><td>3M</td><td>77500</td><td></td></tr><tr><td>Balfour Abdominal Retractor w/ Fixed Side Blades, 4&quot; Deep, 10&quot; Maximum Spread</td><td>MPM Medical Supply</td><td>124-7017</td><td></td></tr><tr><td>Betadine Solution (5% Providone-iodine)</td><td>MWI Animal Health</td><td>NDC-67618-155-01</td><td></td></tr><tr><td>Cefazolin for Injection, USP</td><td>MWI Animal Health</td><td>NDC-63323-237-10</td><td></td></tr><tr><td>Chlorhexidine Solution</td><td>MWI Animal Health</td><td>NDC-30798-624-31</td><td></td></tr><tr><td>Custodial&amp;nbsp;HTK Organ Preservation Solution</td><td>Essential Pharmaceuticals</td><td>25767073545</td><td></td></tr><tr><td>DeBakey Tissue Forceps, 7.75&quot;, 2 mm Tips</td><td>Wexler Surgical</td><td>FL0789.1</td><td></td></tr><tr><td>EUTHASOL (pentobarbital sodium and phenytoin sodium)</td><td>Virbac</td><td>NDC-051311-050-01</td><td></td></tr><tr><td>Heparin Sodium Injection, USP</td><td>MWI Animal Health</td><td>NDC-71288-402-10</td><td></td></tr><tr><td>Hot Dog Temperature Management Controller</td><td>Augustine Surgical Inc.</td><td>WC71V</td><td></td></tr><tr><td>Hot Dog Veterinary Underbody Warming Mattress</td><td>Augustine Surgical Inc.</td><td>V106</td><td></td></tr><tr><td>Invisishield Isolation Bag, 20&quot; x 20&quot;</td><td>Medline</td><td>DYNJSD1003</td><td></td></tr><tr><td>Jacobson Micro Needle Holder, Straight Jaws, Round Handle, 7.25&quot;</td><td>Wexler Surgical</td><td>NL0729.11</td><td></td></tr><tr><td>Ketamine Hydrochloride Injectable Solution</td><td>NexGen Pharmaceuticals</td><td>NC-0256</td><td></td></tr><tr><td>Lap Sponges 18&quot; x 18&quot;</td><td>Medline</td><td>MDS231318LF</td><td></td></tr><tr><td>Metzenbaum Dissection Scissors, 7&quot; Curved</td><td>Wexler Surgical</td><td>SL5011.1S</td><td></td></tr><tr><td>Non-Conductive Suction Tubing with Scalloped Connectors, 1/4&quot; x 10&#039;</td><td>Medline</td><td>DYND50251</td><td></td></tr><tr><td>Pantoprazole Sodium for Injection</td><td>MWI Animal Health</td><td>NDC-55150-202-00</td><td></td></tr><tr><td>Perfusion Cannula, Free-Flow, 3 mm Blunt Tip</td><td>MED Alliance Solutions</td><td>PER-3003S</td><td></td></tr><tr><td>Rigid Bulb Tip Yankauer</td><td>Medline</td><td>DYND50130</td><td></td></tr><tr><td>Satinsky Clamp, 30 mm Angled DeBakey Atraumatic Jaws, Curved Shanks, 10&quot;</td><td>Wexler Surgical</td><td>AL2150.1</td><td></td></tr><tr><td>Scalpel Handle #3</td><td>World Precision Instruments</td><td>500236</td><td></td></tr><tr><td>Servator B UW (University of Wisconsin)</td><td>Global Transplant Solutions</td><td>JFISERB10A r2</td><td></td></tr><tr><td>Single Collection Unit Prefilled CPDA-1, 450 mL</td><td>Jorgensen Laboratories</td><td>JO520</td><td></td></tr><tr><td>Sofsilk Suture Tie, 2-0, Black, 18&quot;</td><td>Covidien</td><td>S-195</td><td></td></tr><tr><td>Surgical Scalpel Blade No. 10</td><td>World Precision Instruments</td><td>500239</td><td></td></tr><tr><td>Surgipro II Suture, 6-0, Blue, 30&quot;, Double Armed, CV-22 Needle</td><td>Covidien</td><td>VP-733-X</td><td></td></tr><tr><td>Three-Quarter Surgical Drape</td><td>Medline</td><td>DYNJP2414</td><td></td></tr><tr><td>Valleylab Electrosurgical Pencil with Stainless Steel Electrodes</td><td>Covidien</td><td>CVNE2516H</td><td></td></tr><tr><td>Valleylab Force FXc Electrosurgical Generator</td><td>Covidien</td><td>MFI-MDT-FORCE-FXC</td><td></td></tr><tr><td>Valleylab Polyhesive Adult Patient Return Electrode</td><td>Covidien</td><td>E7507-SD</td><td></td></tr><tr><td>Xylazine Hydrochloride Injectable Solution</td><td>NexGen Pharmaceuticals</td><td>NC-0334</td><td></td></tr></tbody></table>

kidney procurement in a preclinical large animal model

Kidney Procurement in a Preclinical Large Animal Model

Machine perfusion has evolved as a viable strategy for ex vivo organ assessment, monitoring, treatment, optimization, as well as to prolong preservation times. Large animal models have been paramount for the development and optimization of these technologies. However, in order to ensure graft quality and data reproducibility, standardized and clinically translatable surgical techniques for organ and tissue procurement should be followed. Thus, here, we describe an optimized protocol for kidney procurement in a preclinical swine model. Kidney recovery is performed using mixed breed (Yorkshire cross/mix) pigs. Briefly, following sterile disinfection and draping of the surgical field, a complete midline incision is performed to gain optimal access to both kidneys. The ureter, renal vein, and artery are dissected until their origin from the inferior vena cava and the aorta, respectively. After complete renal dissection, the ureter is tied and cut distally. The donor animal is then fully heparinized with 100 IU per kg/body weight. Next, the renal artery is clamped close to the aorta, and the renal vein is clamped close to the vena cava using a Satinsky vascular clamp. The kidney graft is then resected, and the renal artery is immediately cannulated back table. The kidney will then be flushed with an ice-cold preservation solution and stored on ice until either machine perfusion or transplantation. Finally, the renal artery stump is ligated with a 2-0 silk ligature, and the vena cava is closed with a 6-0 polypropylene suture. This technique recovers kidneys and simulates either a living (single kidney) or deceased (dual kidney) donor setting. The single kidney recovery offers the advantage to perform a subsequent autotransplantation. In the deceased donor model, blood can be collected prior to euthanasia by inserting blood bag needles directly into the aorta, thereby exsanguinating the animal and providing blood for ex vivo machine perfusion.

Our research group has broad experience spanning nearly 15 years with porcine models of both solid organ transplantation and vascularized composite allotransplantation9,10,11,12,13,14,15,16,17. Here, we describe the result...

Here, we present a protocol that details a surgical model for kidney procurement in a preclinical swine model for subsequent machine perfusion or transplantation.

Machine perfusion has evolved as a viable strategy for ex vivo organ assessment, monitoring, treatment, optimization, as well as to prolong preservation ...

Our research aims to develop transformative new tissue and organ preservation techniques, such as using nature-inspired, next-generation cryoprotective agents that allow for ice-free preservation of organs at sub-zero temperatures. Innovative microsurgical small and large animal model are utilized as translational platforms to advance organ and tissue refrigeration, immune modulation, and immune monitoring. Technological advancement are currently transforming the field of transplantation.However, there are remaining challenges in establishing clinically relevant large animal settings to test these technologies in vivo. By extending preservation capabilities from hours to several days, this research has the potential to alleviate one of the most daunting challenges in transplantation, the extremely limited time for organ storage, while also increasing the number of organs available for transplant. To begin, perform a median laparotomy on a surgically prepped anesthetized animal, incising about 25 to 30 centimeters, to gain optimal access to both kidneys.Insert a standard abdominal retractor. Cover the colon and small bowel with towels soaked in warm saline. Retract the bowels to the right for access to the left kidney or to the left for access to the right kidney.Open the peritoneum overlying the kidney and dissect around the kidney to free any adhesions. Dissect the ureter until 10 to 12 centimeters of length is obtained. Next, dissect the renal vein until its origin from the inferior vena cava is exposed.Similarly, dissect the artery until its origin from the aorta is exposed. After complete renal dissection, tie the ureter distally with a 2-0 silk ligature. Cut proximally to the tie, leaving the proximal ureter end open for urine drainage.Administer 100 international units per kilogram of heparin intravenously and wait for two minutes to ensure adequate heparinization of the kidney. Clamp the renal artery close to the aorta using Satinsky vascular clamps. Similarly, clamp the renal vein close to the inferior vena cava.Remove the kidney graft by cutting the renal artery and vein close to the clamps. Flush the kidney with ice-cold University of Wisconsin, or UW, solution or Custodiol histidine-tryptophan-ketoglutarate, or HTK, preservation solution. Remove the perfusion cannula and place the kidney in a sterile organ bag filled with the same ice-cold preservation solution used for flushing.Place the bag within a second sterile organ bag. Ligate the renal artery stump with a 2-0 silk ligature and close the renal vein stump using a two-layer running suture with 6-0 polypropylene. To harvest blood for machine perfusion, identify the infrarenal abdominal aorta.Free any large adhesions or tissue covering the vessel. Insert the blood collection bag needle directly into the aorta. Hang the bag below the animal to facilitate filling.Kidneys were successfully retrieved for transplantation, machine perfusion, and primary cell culture experiments. Notably, successful kidney retrieval was achieved in all experimental groups, implying no complications with this surgical procedure model. The gross appearance of kidney grafts varied at different stages as described in this figure.The native kidney appeared deep red and vascularized. The kidney graft after flushing with ice-cold preservation solution appeared pale. After machine reperfusion, the kidney graft regained a pinkish hue with visible vascular flow.After transplantation, the kidney appears homogeneously perfused, similar to a native kidney. 64%of kidneys exhibit standard anatomy of one renal artery and one renal vein. The most common variations included one artery with two veins, one artery with three veins, two arteries with one vein, and two arteries with two veins.Bilaterally typical anatomy was observed in 46%of pigs while 22%had one atypical kidney on the left, 14%on the right, and 19%had both kidneys atypical.

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JoVE Journal

Medicine

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Kidney Procurement in a Preclinical Large Animal Model

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