Rapid Depletion of Renal Macrophages Using Human CD59/Intermedilysin Cell Ablation Tool

We use intermedilysin mediated oblation of the human CD59 expressing cell in mice to study renal microphage regeneration, focusing on chemokine-driven monocyte recoupment and niche establishment of immune surveillance post injury. Recent development in our field reveal mechanism of renal macrophages regeneration, highlighting a specific chemokine that recruit macrophages and how immune surveillance is reestablished following acute kidney injury. We combine tamoxifen inducible cryogenetics, intermedilysin ablation, multi-parameter flow cytometry, and intravital microscopy to ablate, track, and visualize kidney macrophages in real time.

Key challenges are avoiding systemic inflammation, targeting only resident macrophages, and measuring rapid regeneration without disturbing the delicate renal mIcroenvironment. We showed monocytes restore 88%of kidney macrophages within seven days after ablation, a processes critically dependent on the CX3 CR1, CX3 CL1 signaling axis. To begin, preheat corn oil to 42 degrees Celsius.