Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Dariusz Lenart; Aleksandra Antos; Agata Mitura; Magdalena Staniszewska

doi:10.3791/68204

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Fact...

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Cancer Research

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JoVE Journal Cancer Research

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

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08:28 min

May 9, 2025

DOI: 10.3791/68204-v

Dariusz Lenart¹, Aleksandra Antos¹, Agata Mitura¹, Magdalena Staniszewska¹

¹SDS Optic S.A.

We present a validated sandwich ELISA assay using novel anti-HER2 monoclonal antibodies. This assay enables precise quantification of cell-bound and released HER2 protein from in vitro cultured cells and other samples, including blood and tissues.

We present a validated sandwich, ELISA, using novel monoclonal antibodies, which enables precise quantification of cell bound and released extracellular domain of HER2 protein for in vitro cultured cells and patient samples.

The protocol addresses the need for accurate and validated assay to correlate the concentration of circulating HER2 protein with disease clinical manifestations in diagnostics and research.

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