Normothermic Machine Perfusion of Rat Kidneys for Transplantation

Tong Chen; Kaixin Lan; Haiwei Chen; Li Lin; Yunliang Xie; Shuangjin Yu; Xiaoshun He; Jiang Qiu

doi:10.3791/68251

Normothermic Machine Perfusion of Rat Kidneys for Transplantation

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Medicine
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JoVE Journal Medicine

Normothermic Machine Perfusion of Rat Kidneys for Transplantation

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10:42 min

January 27, 2026

DOI: 10.3791/68251-v

Tong Chen*^1,2,3, Kaixin Lan*^1,2,3, Haiwei Chen*^1,2,3, Li Lin⁴, Yunliang Xie^1,2,3, Shuangjin Yu*^1,2,3, Xiaoshun He*^1,2, Jiang Qiu*^1,2,3,5

¹Organ Transplantation Center,The First Affiliated Hospital of Sun Yat-sen University, ²Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, ³Sun Yat-sen University & Huayue Bio-Artificial Kidney and Renal Regeneration Research Laboratory, ⁴Zhongshan School of Medicine,Sun Yat-sen University, ⁵Yuxi Zhongshan Hospital

This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.

Our laboratory at Sun Yat-sen University has been performing small animal normothermic machine perfusion for five years. This specific protocol outlines critical steps that are necessary for successfully performing NMP and transplantation technique of rat kidneys. To increase the probability and the reproducibility of success, our protocol including three distinctive steps:Acquire the donor kidney, ex vivo NMP, and the subsequent kidney transplantation on the recipient.

Demonstrating this procedure will be Tong Chen. After induction of anesthesia, use an electric clipper to shave the abdominal hair. Range from xiphoid process to the pubic symphysis, both side to the midaxillary line.

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