Name: Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells (Video) | JoVE Journal
Uploaded: 2025-07-03T13:10:00.000+00:00
Duration: 10 min 10 s
Description: Learn to synthesize compound Giant Unilamellar Vesicles as biomimetic models of nucleated cells using electroformation and osmotic shock techniques. This method enables study of electro hydrodynamics and electric field effects on biological membranes.

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5 minutes

Rupesh Kumar thanks the Ministry of Human Resource Development (MHRD), Government of India, for the PMRF fellowship and contingency grant. We gratefully acknowledge the confocal laser scanning microscope of the Central Facility, IRCC, IIT Bombay.

1. Preparation of lipid mixture solutions
<ol>
	<li>Prepare 1 mL solution of DMPC and cholesterol in a 63:37 molar ratio at a final concentration of 5 mg/mL.&#160;For this,&#160;add 149 &#181;L of DMPC (25 mg/mL stock as received dissolved in chloroform), 252 &#181;L of cholesterol (5 mg/mL&#160;cholesterol dissolved in chloroform), and 599 &#181;L of chloroform.</li>
	<li>Prepare another 1 mL solution of DMPC: Cholesterol in a 57:43 molar ratio at 5 mg/mL, mix 139.8 &#181;L of DMPC (25 mg/mL stock as received dissolved in chloroform), 301 &#181;L of cholesterol (5 mg/mL, prepared from powder dissolved&#160;in chloroform), and 559.2 &#181;L of chloroform.</li>
	<li>To stain the lipid bilayer, add 100 &#181;L of Nile Red (from a 5 mM stock solution) to 1 mL of the lipid mixture containing DMPC and cholesterol in a 63:37 molar ratio (described in steps 1.1 and 1.2). Alternatively, add 30 &#181;L of Liss Rhod-PE (1mg/mL stock of Liss Rhod-PE) to the 1 mL of prepared lipid mixture (described in steps 1.1 and 1.2). 
	NOTE: As the solvent (chloroform) is highly volatile, after repeated opening of the vial for use of the stock solution (25 mg/mL of DMPC), it may get&#160;concentrated, which may change the composition.</li>
</ol>
2. Electroformation protocol for synthesizing simple Giant Unilamellar Vesicles (sGUVs)
<ol>
	<li>Thoroughly clean Indium Tin Oxide (ITO) coated slides with a dish soap solution and rinse with deionized water (0.055 &#181;S/cm). Clean the slides with 100% ethanol solution, rinse with deionized water, and dry in an oven at 85 &#176;C.</li>
	<li>Identify the conductive side of the ITO-coated slides using a Clampmeter. Secure the cleaned ITO-coated slide on the spin coater stage using a vacuum with the conductive side facing up.</li>
	<li>Apply 25 &#181;L of the lipid solutions (prepared in step 1.1) by casting four droplets of the solution onto the conductive side of the ITO slide. Then, take another ITO slide and apply 25 &#181;L of the lipid solutions (prepared in step 1.2) by casting four droplets of the solution onto the conductive side of the ITO slide.</li>
	<li>Vacuum-dry both lipid-coated slides in a desiccator that is kept in the dark for at least 2 h. 
	NOTE: Protection from light protects the lipids from possible chemical changes.</li>
	<li>Arrange a lipid-coated ITO slide (coated with lipid prepared in step 1.1) in parallel with other clean ITO slides, conductive side facing each other, with a 3 mm-thick silicone rubber spacer between them. Seal the assembly with a clamp to create a secure electroformation chamber.</li>
	<li>Follow the same procedure described in step 2.5 for the ITO-coated slide with lipid solution prepared in step 1.2 to form an electroformation chamber.</li>
	<li>Place both chambers in an incubator maintained at 38-40 &#176;C and fill the chamber with 100 mM of sucrose solution with the help of a 2 mL syringe. Apply an AC electric field of 5 Vpp at 10 Hz frequency using a dual-channel function generator for 4 h. 
	NOTE: Ensure the ITO slide&#39;s conductive side is correctly clamped to the crocodile clips that connect with the function generator.</li>
	<li>Disconnect the electroformation chamber from the function generator. Remove the chamber from the incubator and allow it to cool to room temperature. Harvest the synthesized sGUVs from both chambers using a syringe and transfer them each into a 2 mL microcentrifuge tube and incubate at room temperature (25-27 &#176;C) for 1 h before subjecting it to osmotic shock.</li>
</ol>
3. Shape transition in osmotic shock (sGUV-to-Stomatocyte-to-cGUV)
<ol>
	<li>Transfer 200 &#181;L of sGUVs suspended in hydrating media containing 100 mM sucrose solution to an observation chamber.</li>
	<li>Introduce 125 &#181;L of glucose solution (300 mM) to this sGUV suspension containing 200 &#181;L of 100 mM sucrose within the observation chamber to induce osmotic shock that initiates the shape transition of the sGUVs. 
	NOTE: Follow the same procedure (given in step 3) for sGUV synthesis from the lipid composition given in steps 1.1 and 1.2 for osmotic shock-driven shape transition.</li>
	<li>Allow the osmotic shock-induced sGUVs to rest for 1 h in the observation chamber placed on the microscopy stage.</li>
	<li>To conduct and observe the shape transition of sGUVS, place the&#160;observation chamber (electrofusion chamber, a Petri dish, a concavity slide, or any other chamber that can be used for microscopy observation) on the stage of an inverted microscope and allow the vesicles to settle.</li>
	<li>Monitor the shape transitions occurring within the chamber. Use Differential Interference Contrast (DIC) and epifluorescence microscopy with Plan fluor, ELWD 20x/0.45, and Plan fluor, ELWD 40x/0.60 objectives lens to observe the shape transitions of the sGUVs.</li>
	<li>Observe the formation of stomatocytic vesicles during transition. Monitor the chamber over time as larger vesicles settle at the bottom, followed by an increased number of smaller vesicles.</li>
</ol>
4. Modifying conductivity in the inner, annular, and outer regions of cGUVs
<ol>
	<li>Adjust the conductivity of solutions in different regions of cGUVs (inner, annular, and outer) by adding appropriate quantities (&#181;L) of a 7.5 mM stock salt solution before&#160;giving an osmotic shock to sGUVs.
	<ol>
		<li>For example, to create higher conductivity in the outer and inner regions compared to the annular region, transfer 200 &#181;L of sucrose solution into the electrofusion chamber. Add 20 &#181;L of 7.5 mM salt solution to the chamber. Induce osmotic shock by adding 125 &#181;L of 300 mM glucose solution.</li>
		<li>Allow the osmotic-shocked sGUVs to rest for 3-4 h.&#160;In this case, cGUVs are obtained with higher conductivity in the outer and inner regions than in the annular region at the end.</li>
	</ol>
	</li>
	<li>Perform electrodeformation of cGUVs by applying an AC electric potential of 7.5 Vpp at 100 kHz using a function generator in an electrofusion chamber that contains wire electrodes spaced 500 &#181;m apart.</li>
	<li>After applying an AC electric field, capture video at 10 frames/s and observe the oblate shape deformation of the outer vesicles and prolate shape deformation of the inner vesicles, confirming higher conductivity in the outer and inner regions than in the annular region in cGUV domains.</li>
</ol>
5. Microscopy analysis of cGUVs
<ol>
	<li>Inverted Microscope Imaging: Perform differential interference contrast (DIC) and epifluorescence microscopy using an inverted microscope and coupled with a monochromatic camera. Use DIC and epifluorescence microscopy with Plan fluor, ELWD 20x/0.45 and Plan fluor, ELWD 40x/0.60 objective lenses to observe the shape transitions of the sGUVs. Use a green filter (EX 510-560, DM 575, BA 590) for the Nile red stain bilayer for the epifluorescence study. 
	NOTE: DIC images provide valuable insights into the status of the connected neck, confirming whether it remains intact or undergoes fusion to form cGUVs.</li>
	<li>Use ImageJ software to insert a scale bar and crop microscopy images. To add a scale bar, go to Analyze | Set Scale to calibrate the image, then go to Analyze | Tools | Scale Bar to insert the scale bar.</li>
	<li>Confocal Microscopy for morphology: To obtain a clearer understanding of the cGUV morphology,&#160;a laser scanning confocal microscope is employed for z-axis scanning with a step size of 1 &#181;m. Imaging with Confocal Microscope: use a laser scanning confocal microscope with a Plan-Apochromat 40x/1.3 oil DIC objective lens to image the cGUVs. Image Nile Red or Rhodamine-PE stained cGUVs using a red single-channel mode, with an excitation wavelength of 561 nm and an emission range of 561-695 nm.</li>
	<li>To modify and extract the .czi file of the image in the microscope-linked software, insert graphics like a scale bar and enable 2D, 3D view, then go to Processing | Method | Parameters and click Apply to get an image in JPG format.</li>
	<li>Preparation for Confocal Microscopy: fill an aqueous solution containing cGUVs in a modified custom chamber (bottom glass replaced with Coverslip glass) for confocal imaging. Leave the sample undisturbed for 10-15 min to allow the cGUVs to settle for confocal imaging; load the cGUVs mixture in the cavity slide and seal with a coverslip to arrest the movements of cGUVs. Following this, analyze the cGUVs through confocal microscopy and z-scanning with 1 &#181;m intervals.</li>
</ol>

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</ol>

The authors have no conflicts of interest to declare.

The composition and types of lipids largely determine the mechanical properties of the bilayer constituting a GUV. A bilayer composed of DMPC/Chol exhibits unique shape transitions when subjected to osmotic shock. Under a strong hypertonic osmotic shock, the aqueous fluid inside the sGUV is expelled, leading to the deformation of the GUV bilayer. The observed shape transitions are influenced primarily by the outward flux of water and the bilayer&#39;s bending rigidity. These deformations manifest in various forms, includ...

Biomimetic models have been extensively employed to study the complex biophysical phenomena of cell membranes. These simplest biomimetic models provide valuable insights by minimizing interference from the myriad variables present in biological cells. Giant Unilamellar Vesicles (GUVs) are a foundational biomimetic system used to study membrane biophysics1,2. GUVs are well established as a representative model of the cell membrane. They comprise a single spherical lipid bilayer of size 10-30 &#181;m in diameter and are encapsulated and suspended in an aqueous solution. In this article, we term these sGUVs. These systems are constructed using a bottom-up approach, where essential cellular components -- lipids, proteins, and catalysts -- are assembled to mimic the structure and function of biological membranes3,4.
Although simple sGUVs, with only the aqueous core inside, are commonly used, more advanced models have been developed to replicate specific features of biological cells. For example, GUVs with actin-supported structures emulate the cytoskeleton5,6,7, while high-salt environments are used to imitate physiological conditions. The higher salt environments mimic physiological conditions but it is difficult to obtain them in GUVs since traditional methods, such as electroformation8,9, are often unsuitable for synthesizing micron-sized GUVs under such conditions. Newer methods, such as the gel-assisted method, which uses polymeric or starch gels to aid bilayer swelling during hydration, address these limitations10,11. Similarly, morphological changes of the sGUVs have been studied12. The addition of closed and open filaments, such as encapsulated actin, microtubules, and other cytoskeletal components, of varying rigidity, and the osmotic stress condition inside them influences the morphological transition of these osmotically deflated but initially quasi-spherical vesicles. Theoretical analysis reveals diverse morphological transformations, including tubular, spherical, racket-like, cherry-like, and droplet structures, aligning well with experimental observations12. These findings have significant implications for understanding the organization of filaments, microtubules, and nanorings within cells and vesicles. Other studies review methods for protein encapsulation and explore how encapsulated actin, microtubules, and cytoskeletal components regulate the shape of the osmotic deflated vesicles12,13.
Further, inspired by eukaryotic cell architecture, complex GUV-based models are developed towards the development of artificial models resembling fission and cell division, reproduction and multicompartment features of eukaryotic cells, self-reproducing cells14,15,16,17, and multicompartment cells. Beyond structural application, GUVs are also the chosen models for understanding cell electroporation -- a process where an applied electric field induces pore formation in the lipid membrane due to Maxwell stress. GUVs, with dimensions comparable to biological cells, exhibit electrodeformation and electroporation under electric fields. Such studies on GUVs provide insights into electromechanical properties such as membrane capacitance and bending rigidity18 and into electroporation mechanisms2,19,20. However, practically, these studies are best representative of anucleate cells, such as red blood cells (RBCs).
To bridge this gap, we propose compound Giant Unilamellar Vesicles (cGUVs) as a novel biomimetic model for nucleate eukaryotic cells21,22. These vesicles possess a vesicle-in-vesicle structure, where the inner bilayer mimics the nuclear membrane, and the outer bilayer represents the plasma membrane. Furthermore, we demonstrate how the electrical conductivity of the inner, annular, and outer regions can be modulated without requiring advanced instrumentation, such as micromanipulators or microsyringes. This simplified approach to synthesizing cGUVs offers a promising platform for advancing biophysical research on nucleated cells.

<table><tbody><tr><td>Cholesterol (Sigma grade , 99%)</td><td>Avanti Polar Lipids, Inc.</td><td>C8667</td><td>For sGUV synthesis</td></tr><tr><td>Choloroform</td><td>Merck Life Science Pvt. Ltd.</td><td>DA2P711602</td><td>used for solvent for preparation of lipid stock solution</td></tr><tr><td>DMPC</td><td>Avanti Polar Lipids, Inc.</td><td>850345C</td><td>For sGUV synthesis</td></tr><tr><td>Ethanol</td><td>Hayman</td><td>F204325</td><td>For cleaning of ITO slides</td></tr><tr><td>FITC-DEXTRAN (4 Kda-MW)</td><td>Sigma-Aldrich</td><td>466994</td><td>For encapsulation inside the inner vesicles of cGUV</td></tr><tr><td>Glucose</td><td>Sigma-Aldrich</td><td>G-7021</td><td>For preparation of glucose solution, used for osmotic shock to sGUV suspended in the sucrose solution</td></tr><tr><td>Liss Rhod PE</td><td>Sigma-Aldrich</td><td>810150C-1MG</td><td>To stain a lipid molecules</td></tr><tr><td>Nile red</td><td>Sigma-Aldrich</td><td>72485</td><td>For staining of cGUV and sGUV membrane</td></tr><tr><td>Sodium chloride (NaCl)</td><td>Sigma-Aldrich</td><td>1936060521</td><td>For preparation of salt stock solution, to change the conductivity&amp;nbsp; of the cGUV compartments</td></tr><tr><td>Sucrose</td><td>Sigma-Aldrich</td><td>S9378</td><td>For sucrose sol. as a hydrating media of the electroformation chamber</td></tr><tr><td>Equipments</td><td></td><td></td><td></td></tr><tr><td>Cavitly slides</td><td>Blue star</td><td></td><td>cGUV suspension placed for microscopy observation</td></tr><tr><td>cMOS pco.edge 4.2 camera</td><td>Excelitas</td><td>61009524</td><td>For fluorescent monochromatic&amp;nbsp; images and DIC imaigng</td></tr><tr><td>Cover slip (22 x 60 mm)</td><td>Blue star</td><td></td><td>For covering of cavity slides</td></tr><tr><td>Eppendrof Electrofusion chamber</td><td>Sigma-Aldrich</td><td></td><td>sGUV suspension placed in the chamber for osmotic and microscopy observation, any other simple chamber can also be used</td></tr><tr><td>Function generator (Dual channel)</td><td>Tektronix</td><td>AFG3022C</td><td>Function generator used to deliver AC electric field to electroformation&amp;nbsp; chamber</td></tr><tr><td>Incubator</td><td>local customised</td><td></td><td>electroformation chamber placed at 40?</td></tr><tr><td>ITO slides</td><td>Sigma-Aldrich</td><td>576352-10PAK</td><td>ITO slides used in electroformation method</td></tr><tr><td>Nikon Eclipse inverted micrscope</td><td>Nikon</td><td>TE2000-U</td><td>For visulissation of sGUV and cGUV</td></tr><tr><td>Silicone sheet (3 mm&amp;nbsp; thickness)</td><td>Sigma-Aldrich</td><td>GF49463141</td><td>For spacing in electroformation chamber</td></tr><tr><td>Syringe (2 mL)</td><td>Becton Dickinson India Pvt Ltd.</td><td>300844</td><td>Used in injecting a hydrating media and harvesting the sGUV from electroformation chamber</td></tr><tr><td>Zeiss confocal microscopy setup</td><td>Central facility of IIT Bombay</td><td></td><td></td></tr></tbody></table>

synthesis of compound giant unilamellar vesicles: a biomimetic model of nucleate cells

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

A simple Giant unilamellar vesicle (sGUV) is a micron-sized spherical vesicle, which is composed of a lipid bilayer that encloses an aqueous solution inside and is suspended in another aqueous medium. As biomimetic models of biological cells, sGUVs are well-established systems for biophysical studies. However, sGUVs primarily mimic anucleate cells, limiting their application as models for non-nucleate cells. We propose compound Giant Unilamellar vesicles (cGUVs) as a more appropriate biomimetic model for nucleated cells. cGUVs are vesicle-in-vesicle structures, where the outer and inner vesicle bilayers can be assumed to represent the cell and the nuclear membrane of a cell, respectively.

In this study, we describe a simple method for the synthesis of cGUVs. Briefly, sGUVs were generated using the lipid composition of DMPC and cholesterol within the range of 37-43 mol% in a sucrose medium via the electroformation method. These sGUVs were then subjected to osmotic shock by introducing a hypertonic glucose solution, triggering an immediate transition to a somatocytic shape. This process led to the formation of an intermediate state where the outer and inner vesicles remained connected through a neck, ultimately resulting in the development of vesicle-in-vesicle structure, referred to as cGUVs.
Furthermore, it is known that GUVs prepared with higher cholesterol levels in the bilayer exhibit reduced ion permeability. This characteristic allows tuning of conductivity in the annular solution of a cGUV by adjusting the conductivities of the hydrating medium,&#160;the inner solution through hypertonic glucose, and the outer solution by adjusting of the&#160;dilution or the addition of conductive media, making the resultant cGUVs with controlled conductivities in the outer, annular, and inner regions, relevant for electric field studies. We propose this efficient and straightforward approach for synthesizing compound vesicles that can be used as a biomimetic model for eukaryotic, nucleated cells, advancing their application in biophysical research.

sGUVs were synthesized using the electroformation method (as shown in Figure 1) and harvested from the chamber using a syringe. The harvested sGUVs were initially visualized under an inverted microscope and subjected to an osmotic shock to induce their transformation into a stomatocyte shape. After further membrane remodeling, this stomatocyte is transformed into a vesicle-in-vesicle structure called a compound Giant Unilamellar Vesicle (cGUV) (Figure 1). The cG...

This article presents a method for preparing compound Giant Unilamellar Vesicles (cGUVs) with a vesicle-in-vesicle structure, with customized electrical conductivity in inner, annular, and outer regions. Electroformation synthesizes simple GUVs, which are transformed into stomatocytes and cGUVs via osmotic shock, providing a valuable model for studying the biophysics of nucleated cells.

A simple Giant unilamellar vesicle (sGUV) is a micron-sized spherical vesicle, which is composed of a lipid bilayer that encloses an aqueous solution ...

We've conducted an elaborate study on the synthesis and electro hydrodynamics of compound giant unilamellar vesicles to establish them as biomagnetic equivalents of eukaryotic cells. The attempt is in understanding technologies involving application of electric field to biological cells such as cell electroporation and cell electrodeformation.A combination of a fluorescence and light microscopy, nanosecond pulse electric treatments, oscilloscope and power sources, and innovative synthesis methods are employed to advance the research in this area. The challenge in the synthesis of well-formed compound GUVs in the sensitivity of a method to a temperature, types of lipids, and the composition used. In this work, we demonstrate this for a DMPC and cholesterol system, but generalizing it remains challenging. Simple GUVs that mimic a nucleic cells have been synthesized in the literature. We synthesize the compound giant vesicle to emulate a nucleated cells with the valve defense structure, enclosing a inner vesicle of roughly half of the size of the outer vesicle.We will focus on studying the effect of micro and nanosecond pulses on the inner vesicle, which is a mimic for the nucleus of a biological cell in the context of electroporation.[Narrator] To begin, thoroughly clean the indium tin oxide or ITO-coated slides using a dish soap solution, and rinse with deionized water with a conductivity of 0.055 microsiemens per centimeter. Then using a 100% ethanol solution, clean the slides again and rinse with deionized water. Dry the slides in an oven set to 85 degrees Celsius. Identify the conductive side of each ITO-coated slide using a clamp meter. Secure the clean slide onto the spin coder stage using a vacuum, ensuring that the conductive side is facing upward. Apply 25 microliters of the lipid solution, one is four droplets onto the conductive side of one ITO slide. Take another slide and apply 25 microliters of the lipid solution two in the same manner. Vacuum dry both lipid coated slides in a desiccator stored in the dark for a minimum of two hours. Then arrange a lipid-coated ITO slide with lipid one in parallel with a clean slide, ensuring the conductive sides face each other, and place a three-millimeter thick silicone rubber spacer between them. Seal the setup with a clamp to create an electroformation chamber. Now fill the chamber with 100-millimolar sucrose solution using a two-milliliter syringe. Connect the output cable of the function generator to the ITO-coated slides with the function generator. Place both electroformation chambers in an incubator maintained between 38 and 40 degrees Celsius. Apply an alternating current electric field of five volts peak-to-peak at 10 hertz frequency using a dual-channel function generator for four hours. After incubation, disconnect the electroformation chambers from the function generator. Remove them from the incubator, and allow them to cool to room temperature. Using a syringe, harvest the synthesized giant unilamellar vesicles from each chamber and transfer them into separate two-milliliter microcentrifuge tubes. Incubate the tubes at room temperature between 25 and 27 degrees Celsius for one hour before proceeding to osmotic shock. Transfer 200 microliters of simple giant unilamellar vesicles, or sGUVs, suspended in hydrating media containing 100-millimolar sucrose solution into the observation chamber, and introduce 125 microliters of 300-millimolar glucose solution to induce osmotic shock and initiate shape transitions. Allow the sGUVs, now undergoing osmotic shock, to rest for one hour inside the observation chamber placed on the microscopy stage. Perform differential interference contrast and epifluorescence microscopy using an inverted microscope equipped with a monochromatic camera. Use Plan Fluor extra-long working distance 20X by 0.45 and 40X by 0.60 objective lenses to observe shape transitions in the sGUVs. For epifluorescence, use a green filter set with excitation at 510 to 560 nanometers, a dichroic mirror at 575 nanometers, and a barrier filter at 590 nanometers for Niall Red-stained bilayers. Monitor shape transitions within the observation chamber using differential interference contrast and epifluorescence microscopy with Plan Fluor objective lenses. Observe the formation of stomatocytic vesicles as the shape transition proceeds. Monitor how the larger vesicles settle first, followed by an increase in the number of smaller vesicles over time. Next, add salt solution to adjust conductivity in different regions of the cGUVs before inducing osmotic shock. For example, to create higher conductivity in the outer and inner regions than the annular region, transfer 200 microliters of sucrose solution into the electrofusion chamber. Add 20 microliters of 7.5-millimolar salt solution into the chamber, and induce osmotic shock with 125 microliters of 300-millimolar glucose solution. Allow the osmotic shock sGUVs to rest in the chamber for three to four hours. Confirm that the resulting cGUVs have higher conductivity in the outer and inner regions compared to the annular region. To perform electrodeformation of the cGUVs, space the wire electrodes 500 micrometers apart, and apply an alternating current electric potential of 7.5 volts peak-to-peak at 100 kilohertz using a function generator. After the electric field is applied, capture video at 10 frames per second, and observe the oblate deformation of the outer vesicles and the prolate deformation of the inner vesicles. Then load the cGUV mixture into a cavity slide and seal with a cover slip to prevent movement. Use a laser scanning confocal microscope to analyze cGUV morphology through Z-axis scanning with a step size of one micrometer. Employ a Plan Apochromat 40X by 1.3 oil DIC objective lens for imaging. Use a red single-channel mode with 561 nanometers excitation and 561 to 695 nanometers emission to image cGUV stained with Niall Red or Rhodamine PE. Modify and extract the .CZI image file using the microscope-linked software. Insert graphics like scale bars, and enable 2D and 3D views. Then go to processing method and parameters to adjust the desired settings. Then click Apply to export the image in JPG format. Finally, open the file in ImageJ software. To insert a scale bar, go to analyze, followed by set scale to calibrate, then select analyze followed by tools and scale bar to apply it. Confocal Z-stack imaging confirmed that inner vesicles were fully separated from the outer vesicles and elevated in the Z plane due to lower density of the inner solution. The intermediate stomatocyte state showed a narrow neck connecting inner and outer vesicles before complete separation. A diverse population of vesicular forms, including multiple inner vesicles, star-shaped bodies, and tubular structures were observed six hours after osmotic shock. A high abundance of cGUVs was formed using a lipid mixture of 1,2-Dimyristoyl-sn-glycero-3-phosphocholine and cholesterol in a 63 to 37 molar ratio. Under an alternating current electric field, cGUVs showed deformation with the outer vesicle forming an oblate shape and the inner vesicle forming a prolate shape.

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Research

JoVE Journal

Bioengineering

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