Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds

Here, we present a protocol to optimize asymbiotic seed germination, seedling development, and shoot elongation in Hemipilia cucullata. The protocol also enables the induction of protocorm-like bodies from sterile leaf explants, facilitating efficient in vitro propagation for the conservation and sustainable use of this endangered medicinal orchid.