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Chapters
- 00:01Title
- 00:55Introduction
- 01:41Labeling of Human Mesenchymal Stem Cells with Fluorescent Dye DiD
- 03:12Labeling of Human Embryonic Stem Cells with the Fluorescent Dye ICG
- 06:23Imaging Stem Cells in vivo
- 06:59Conclusion
Questo video mostra le tecniche per l'etichettatura delle cellule staminali embrionali umane e cellule staminali mesenchimali con coloranti fluorescenti. Questa tecnica può essere utilizzata per un monitoraggio in vivo di cellule staminali trapiantate con imaging ottico e per le correlazioni istopatologiche con microscopia a fluorescenza.
Tags
LabelingStem CellsFluorescent DyesNon-invasive DetectionOptical ImagingIn Vivo MonitoringEx Vivo LabelingIntravenous InjectionVisualizationTarget OrgansPathologiesHuman Mesenchymal Stem Cells (hMSC)Lipophilic Fluorescent Dye DiDHuman Embryonic Stem Cells (hESC)FDA Approved Fluorescent Dye Indocyanine Green (ICG)Uptake MechanismAdherenceDiffusionPhospholipid Cell Membrane BilayerLabeling EfficiencySerum-free MediaSerum-containing MediaTransfection Agent Protamine SulfateContrast Agent Uptake
