Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein

Neutrophil extracellular traps are involved in a variety of diseases, including cardiovascular diseases. However, the synergistic effects of NET formation and lipoproteins on vascular cells remain unclear. Utilizing this protocol, mechanistic analysis are ongoing to this date how inflammatory responses are induced in human aortic endothelial cells treated with neutrophil extracellular traps and low-density lipoprotein.

We have demonstrated that high-density lipoprotein acts as a suppressor of NET formation. That is promoted by oxidized low-density lipoprotein, oxidized phospholipids, and lysophospholipids. To begin, centrifuge 45 to 50 milliliters of human whole blood obtained in a heparin tube at 700 G for 15 minutes at four degrees Celsius.