A Refined Aerosol-Based Intratracheal Bleomycin Delivery Method for Reproducible and Minimally Invasive Mouse Models of Pulmonary Fibrosis

We aim to develop a reproducible, minimally invasive, aerosol-based, drug delivery model for inducing pulmonary fibrosis and improving preclinical drug evaluation. Our model enables consistent and reliable drug delivery while reducing surgical trauma and improving reproducibility across experiments. We plan to apply this model to test our unique therapy for pulmonary fibrosis and a clinical lung injury.

To begin, using a sterile, one-milliliter syringe, withdraw three milliliters of sterile 0.9%sodium chloride, then inject sodium chloride into a vial containing 15 units of bleomycin hydrochloride. Gently swirl the vial until the bleomycin powder is fully dissolved to obtain a five-units-per-milliliter stock solution. Aliquot the stock solution into sterile, 1.5-milliliter micro centrifuge tubes.