Functional constipation is commonly encountered in adult healthcare, yet its clinical assessment is often limited by inconsistent recognition, insufficient nurse training, and the lack of a standardized instrument to assess how healthcare professionals integrate knowledge, attitudes, and practices into routine care. To address this gap, this study developed and psychometrically evaluated the Knowledge-Attitude-Practice Questionnaire for Health Professionals in Adult Functional Constipation Care (KAP-FC). Guided by the Knowledge-Attitude-Practice (KAP) framework, an initial item pool was generated through a literature review, theoretical analysis, and qualitative interviews, followed by two rounds of Delphi consultation with an interdisciplinary expert panel. The revised questionnaire was pre-tested and subsequently administered to 466 nurses from three tertiary hospitals in Nantong using stratified convenience sampling. Item screening was performed using the critical ratio method and item-total correlation analysis, while structural validity, content validity, internal consistency, and test-retest reliability were evaluated. Following item analysis, 28 items were retained, comprising 12 knowledge, 6 attitude, and 10 practice items. Exploratory factor analysis identified six factors that explained 72.344% of the total variance, with factor loadings ranging from 0.511 to 0.894. The overall Cronbach's α was 0.914, with dimension-specific Cronbach's α values ranging from 0.881 to 0.900. The intraclass correlation coefficient for test-retest reliability was 0.936, and the scale-level content validity index (S-CVI) was 0.929. These findings demonstrate that the KAP-FC questionnaire has satisfactory preliminary reliability and validity and may serve as a useful instrument for assessing healthcare professionals' knowledge, attitudes, and practices related to adult functional constipation care.