The presented protocol aims to develop a cellular model that mimics human obesity. To achieve this, human adipose-derived stem cells are differentiated into adipocytes, which are next fattened using a mixture of selected fatty acids.
Method Article
Access restricted. Please log in or start a trial to view this content.
Access restricted. Please log in or start a trial to view this content.
Access restricted. Please log in or start a trial to view this content.
Access restricted. Please log in or start a trial to view this content.
Access restricted. Please log in or start a trial to view this content.
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 3-Isobutyl-1-methylxanthine (IBMX) | Merck | I5879 | |
| 0.25% trypsin/0.05% EDTA pH 7.2 | IITD PAN, Wroc aw, Poland | 20-500 | |
| 96-well plate | Thermo | 130188 | |
| antibiotics-antimycotic solution | Sigma-Aldrich | A5955 | |
| Bovine Serum Albumin (BSA) | Sigma-Aldrich | A3294 | |
| Cell counter | N/A | N/A | |
| Centrifuge | N/A | N/A | |
| Dexamethasone | Sigma-Aldrich | D4902 | |
| Dulbecco's Modified Eagle Medium (DMEM) | IITD PAN, Wroc aw, Poland | 10-500 | containing 1 g/L glucose |
| Dulbecco's Modified Eagle Medium:Nutrient Mixture F12 | Gibco | 11320-033 | |
| Ethanol | Chempur | 113964200#1L | |
| Fetal bovine serum (FBS) | Gibco | A5256701 | |
| hADSC Human Adipose-Derived Stem Cells | Lonza | PT-5006 | |
| Incubator | N/A | N/A | |
| Indomethacin | Sigma-Aldrich | I8280-5G | |
| Insulin | Sigma-Aldrich | I9278 | |
| Inverted microscope | N/A | N/A | |
| L-glutamine | Sigma-Aldrich | 25030-024 | |
| Linoleic acid sodium salt | Sigma-Aldrich | L8134 | |
| LipidSpot fluorescent | Biotium | 70065 | |
| Phosphate-buffered saline (PBS) | Thermo | 10010023 | |
| Soduim oleate | Sigma-Aldrich | O7501 | |
| Soduim palmitate | Sigma-Aldrich | P9767 | |
| T75 cell culture flask | Thermo | 130190 | |
| Trypan blue | Sigma-Aldrich | 95896HJ |
Access restricted. Please log in or start a trial to view this content.
Request permission to reuse the text or figures of this JoVE article
Request Permission