Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

RNA secondary structure has primarily been observed in mature RNA with structure probing methods. Co-transcriptional Structure Tracking sequencing (CoSTseq) unifies nuclear run-on, which has been used to study Polymerase position on nascent RNA, with structure probing. CoSTseq thereby enables observation of RNA secondary structure in RNA under active transcription.