Effectiveness of Topical Huzhang Sanhuang with Standard Nursing for Chemotherapy-Induced Phlebitis: Randomized Controlled Study

Summary

This randomized controlled study evaluates whether the topical Huzhang Sanhuang formula added to standard nursing care improves symptom resolution, inflammation, and safety outcomes in patients with chemotherapy-induced phlebitis.

Abstract

Chemotherapy-induced phlebitis (CIP) is a common complication of peripheral intravenous chemotherapy that can cause pain, local inflammation, treatment interruption, and diminished quality of life. This randomized controlled trial evaluated the efficacy and safety of the topical Huzhang Sanhuang (HZSH) formula, combined with standard nursing care, in managing CIP. Ninety-four hospitalized patients with CIP (grade I or higher) were randomly assigned in a 1:1 ratio to receive either topical HZSH formula plus standard nursing care (experimental group, n = 47) or 50% magnesium sulfate wet dressing plus standard nursing care (control group, n = 47) for 14 days. Prespecified outcomes included phlebitis grade, visual analog scale (VAS) pain score, high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), symptom resolution time, and safety indicators. By Day 14, patients in the experimental group demonstrated significantly greater improvement in phlebitis severity than those in the control group (risk ratio for grade ≥ II, 0.42; 95% confidence interval, 0.20–0.87; P = 0.012). The experimental group also showed significantly larger reductions in VAS pain scores, hs-CRP levels, and IL-6 concentrations (all P < 0.01). Kaplan–Meier analysis further demonstrated faster resolution of multiple local symptoms in the experimental group. Treatment adherence was high in both groups, and no serious adverse events or major safety concerns were observed. These findings indicate that the topical HZSH formula, combined with standard nursing care, is a safe and effective integrative nursing intervention that accelerates clinical recovery, alleviates local symptoms, and reduces the inflammatory burden in patients with chemotherapy-induced phlebitis.

Introduction

Chemotherapy-induced phlebitis (CIP) is one of the most common complications in patients with malignancies receiving peripheral intravenous chemotherapy¹. Although central venous catheters and implantable ports are increasingly used in oncology care, peripheral venous access remains common in short-course chemotherapy and primary healthcare settings because of cost constraints, procedural convenience, and patient-specific vascular conditions. In this setting, cytotoxic agents such as vincristine, oxaliplatin, and docetaxel may cause substantial endothelial injury due to high osmolarity, pH imbalance, and direct vascular toxicity, thereby triggering local inflammatory responses^2,3. Clinically, CIP may present with pain, erythema, swelling, and induration; in severe cases, it may progress to local tissue necrosis. These complications may interrupt treatment schedules, delay antitumor therapy, and increase patient distress related to repeated puncture and vascular injury, ultimately reducing quality of life⁴.

Current nursing management of CIP mainly follows intravenous infusion therapy standards and routine supportive care, including limb elevation, local cold or warm compresses, and standardized management of peripheral indwelling needles⁵. Common topical interventions include magnesium sulfate wet dressings, mucopolysaccharide polysulfate ointment, and hydrocolloid dressings^6,7. However, these approaches have practical limitations. Magnesium sulfate dressings may reduce edema through osmotic effects, but they often require frequent replacement and may cause skin dryness or pruritus. Conventional topical agents may improve local circulation to some extent, but their effects are often limited in patients with marked burning pain, persistent induration, or more severe inflammatory reactions. Available evidence also suggests that single-modality nursing measures or topical chemical agents may be insufficient to fully support endothelial recovery and reduce downstream vascular complications, including thrombotic risk^8,9. Therefore, a practical, clinically applicable integrated nursing strategy is needed to reduce inflammation, relieve pain, and support local vascular repair¹⁰.

In traditional Chinese medicine (TCM), chemotherapy-associated vascular injury is commonly understood as a condition involving heat-toxin accumulation and blood stasis, with local vessel damage and impaired circulation, as key pathological features^11,12. The Huzhang Sanhuang formula includes herbs such as Polygonum cuspidatum, Rheum palmatum, Phellodendron amurense, and Scutellaria baicalensis, and is traditionally used for heat-clearing, detoxification, blood-activating, stasis-dispersing, swelling reduction, and pain relief. Modern pharmacological studies provide a plausible biological basis for these effects. Active constituents in Polygonum cuspidatum (including resveratrol-related compounds) have been reported to suppress inflammatory signaling and reduce oxidative stress-associated endothelial injury, while Rheum palmatum may support local microcirculation and tissue repair¹³. In addition, compounds from Phellodendron amurense and Scutellaria baicalensis, such as berberine and baicalin, have been documented to have anti-inflammatory and antimicrobial effects. When used topically, this herbal compound formulation may help establish a localized anti-inflammatory microenvironment at the lesion site while avoiding systemic adverse effects associated with certain oral or injectable medications¹⁴.

Against this background, the present randomized controlled study was designed to evaluate the clinical effectiveness and safety of the topical Huzhang Sanhuang formula combined with standard nursing care in hospitalized patients with CIP¹⁵. Patients were randomly assigned to a control group receiving standard nursing care plus routine topical treatment, or to an experimental group receiving the Huzhang Sanhuang topical formula in addition to the same standard nursing care. The primary expectation was that the combined intervention would reduce the severity of phlebitis and accelerate the resolution of pain, redness, and swelling. By using a controlled design and prespecified outcome measures, this study aims to provide stronger evidence for an integrated nursing approach to CIP and to support the standardized application of TCM-based topical care in modern oncology supportive nursing practice.

Protocol

Approval was obtained from the Ethics Committee of the Second Affiliated Hospital of Nanjing University of Chinese Medicine (Jiangsu Provincial Second Traditional Chinese Medicine Hospital) before initiating participant recruitment (Approval no. 2025SEZKY-033-02). All study procedures were conducted in accordance with the Declaration of Helsinki. The study purpose, procedures, potential benefits, and possible risks were explained to each eligible participant, and written informed consent was obtained before enrollment.

1. Protect participant confidentiality

1. Assign study identification codes
   1. Assign a unique study identification code to each participant immediately after enrollment. Use the assigned code throughout the study.
   2. Enter the study identification code on all case report forms, intervention records, laboratory records, and study databases. Do not record participant names in the analysis dataset.
2. Secure personally identifiable information
   1. Store participant names, contact information, hospital identification numbers, and consent forms separately from coded research data. Link identifiable information to the study identification code only through a secure participant-code list.
   2. Limit access to personally identifiable information to authorized research personnel. Store paper records in locked cabinets and protect electronic records with passwords and access controls.
   3. Do not disclose participant-identifying information in study reports, statistical outputs, presentations, or publications. Use coded or aggregated data when reporting study findings.
3. Document study events prospectively
   1. Document each protocol deviation when it occurs. Record the date, nature, reason, corrective action, and effect on study participation.
   2. Document each participant's withdrawal prospectively in the case report form and study log. Record the withdrawal date and reason without altering previously collected data.
   3. Record all adverse events in the case report forms and nursing records. Document the onset, severity, duration, management, outcome, and assessed relationship to the study intervention.

2. Study design and participant enrollment

1. Study design
   1. Conduct a prospective, randomized, controlled, single-center clinical trial at the Second Affiliated Hospital of Nanjing University of Chinese Medicine from January 2025 to December 2025. Compare topical Huzhang Sanhuang formula plus standard nursing care with 50% magnesium sulfate wet dressing plus the same standard nursing care.
   2. Initiate the assigned intervention immediately after confirming chemotherapy-induced phlebitis (CIP) and completing baseline assessment. Continue treatment for 14 consecutive days and perform outcome assessments on Day 0, Day 7, and Day 14.
   3. Identify hospitalized patients who develop CIP after peripheral intravenous chemotherapy during routine chemotherapy infusion rounds and intravenous therapy rounds. Evaluate each patient for study eligibility.
   4. Explain the study objectives, procedures, risks, and benefits to each eligible participant. Obtain written informed consent before enrollment.
   5. Collect demographic information, tumor treatment status, phlebitis grade, visual analog scale (VAS) pain score, and inflammatory biomarker measurements before randomization.
2. Participant screening
   1. Calculate the sample size using the primary endpoint, defined as the proportion of participants with persistent grade II or higher phlebitis at Day 14. Assume an event rate of 45% in the control group and 15% in the experimental group with a two-sided α of 0.05 and 80% statistical power.
   2. Require at least 36 participants per treatment group based on the sample size calculation. Increase the target sample size by approximately 20% to compensate for anticipated withdrawals or protocol deviations and enroll 94 participants (47 per group).
   3. Include adults aged 18 years or older who develop grade I or higher phlebitis at the peripheral intravenous catheter insertion site during chemotherapy. Confirm that each participant is conscious, capable of reporting symptoms, and able to complete scheduled follow-up assessments.
   4. Obtain written informed consent from each eligible participant before enrollment. Assign each participant a unique study identification number.
   5. Exclude patients with a known allergy to either topical preparation, severe contact dermatitis, skin ulceration or infection at the treatment site, severe eczema, or extensive local skin damage. Exclude patients with severe systemic infection, unstable organ failure, cognitive impairment, communication difficulties, or concurrent topical treatments that could influence study outcomes.
   6. Withdraw participants who revoke consent, miss more than 20% of scheduled topical applications, or receive prohibited topical medications during the study. Withdraw participants who develop severe local skin reactions requiring discontinuation or who cannot complete follow-up because of transfer or hospital discharge.
   7. Record the reason, date, and timing of each withdrawal in the study records. Document all protocol deviations and adverse events before study completion.

3. Randomization, allocation concealment, and blinding

1. Randomization and allocation concealment
   1. Ask an independent researcher who is not involved in participant recruitment, intervention delivery, or outcome assessment to generate a computer-based random allocation sequence before participant enrollment. Allocate participants to the experimental or control group in a 1:1 ratio.
   2. Place each treatment assignment in a sequentially numbered, opaque, sealed envelope. Seal each envelope securely before participant enrollment begins.
   3. Ask trained research nurses to screen potential participants according to the eligibility criteria. Confirm eligibility and obtain written informed consent before enrollment.
   4. Collect all baseline demographic and clinical data before revealing the treatment allocation. Verify that all required baseline assessments have been completed.
   5. Ask the study coordinator to open the next sequentially numbered envelope only after participant enrollment and completion of the baseline assessment. Record the envelope number and treatment assignment in the study records.
   6. Communicate the assigned treatment to the intervention nurse immediately after opening the allocation envelope. Begin the assigned intervention according to the study schedule.
   7. Record the allocation date, envelope sequence number, assigned treatment group, and responsible study personnel in the study documentation.
2. Blinding and bias control
   1. Assign different study personnel to deliver the topical interventions and perform outcome assessments. Prevent outcome assessors from participating in treatment administration.
   2. Use identical assessment forms and standardized evaluation procedures for all scheduled study visits. Apply the same assessment criteria to both treatment groups.
   3. Keep outcome assessors unaware of participant treatment allocation whenever feasible. Avoid discussing treatment assignments during outcome assessments.
   4. Replace treatment groups with coded identifiers before statistical analysis. Keep the treatment code concealed until completion of the primary statistical analyses.
   5. Perform all efficacy and safety assessments according to the predefined protocol. Record all observations immediately after each assessment without reference to previous results.
      NOTE: Do not blind participants because the Huzhang Sanhuang formula and 50% magnesium sulfate dressing differ in appearance, texture, and odor. Limit blinding to outcome assessment and statistical analysis whenever feasible.

4. Topical intervention procedures

1. Overall intervention framework
   1. Provide all participants with the same standard nursing care throughout the study. Vary only the assigned topical treatment between the two groups.
   2. Begin the assigned topical treatment immediately after confirming grade I or higher chemotherapy-induced phlebitis and completing the baseline assessment. Continue treatment for 14 consecutive days.
   3. Administer the assigned topical treatment twice daily, once in the morning and once in the evening, at approximately fixed times each day. Where clinically feasible, maintain an interval of approximately 12 h between the two applications. Perform outcome assessments on Day 0, Day 7, and Day 14 before the morning application.
   4. Standardize treatment delivery across participants by using the same predefined procedures for skin preparation, treatment coverage, dressing retention, documentation, and safety monitoring. Maintain an application thickness of approximately 2 mm for the Huzhang Sanhuang paste, extend both topical treatments approximately 1 cm beyond the visible lesion margin, and leave each dressing in place for 4 h. Record the actual application and removal times after each treatment.
2. Prepare the Huzhang Sanhuang formula
   1. Obtain 100 g each of Polygonum cuspidatum (Huzhang), Rheum palmatum (Dahuang), Phellodendron amurense (Huangbai), and Scutellaria baicalensis (Huangqin). Verify each herb according to the Chinese Pharmacopeia before preparation.
   2. Record the supplier, batch number, authentication result, and quality certificate for each herbal ingredient before use.
   3. Add 4,000 mL of purified water to the herbal mixture at a crude herb-to-water ratio of 1:10 (w/v). Soak the mixture for 30 min before extraction.
   4. Heat the soaked herbal mixture to gentle boiling (approximately 95–100 °C). Continue decoction for 30 min.
   5. Filter the decoction through an appropriate filter. Concentrate the filtrate at 70–80 °C until a homogeneous, semisolid paste suitable for topical application is obtained.
   6. Inspect the paste for uniform appearance and absence of visible contamination or phase separation. Confirm that the paste has a consistent semisolid consistency suitable for topical application.
   7. Transfer the paste into sterile sealed containers. Store the preparation at 2–8 °C and prepare a fresh batch every 48 h.
   8. Apply approximately 0.20 g/cm² of herbal paste to achieve a layer approximately 2 mm thick. Extend the application approximately 1 cm beyond the visible lesion margin.
3. Administer the Huzhang Sanhuang formula (Experimental group)
   1. Inspect the affected skin before each application. Assess erythema, swelling, pain, papules, blistering, exudation, and pruritus.
   2. Clean the treatment site gently with sterile normal saline. Dry the skin using sterile gauze before applying the herbal paste.
   3. Spread the Huzhang Sanhuang paste evenly over the inflamed area to a thickness of approximately 2 mm. Extend the application approximately 1 cm beyond the visible lesion margin.
      NOTE: Do not apply pressure directly over the inflamed vein during application. Ensure complete coverage of the affected area.
   4. Cover the treated area with sterile gauze. Secure the dressing using hypoallergenic tape without applying compression.
   5. Leave the topical preparation in place for 4 h. Remove the dressing at the end of the treatment period.
   6. Remove any remaining herbal paste with sterile normal saline. Dry the skin gently using sterile gauze.
   7. Repeat the application twice daily for 14 consecutive days. Administer treatments at approximately the same time each day.
   8. Record the application time, treatment completion, skin tolerance, and protocol deviations after each application. Report any clinically significant local reactions immediately.
4. Administer the control treatment
   1. Inspect, clean, and dry the affected area using the same procedure applied to the experimental group.
   2. Soak sterile gauze thoroughly with 50% magnesium sulfate solution. Remove excess solution before application.
   3. Place the soaked gauze over the inflamed area. Extend the dressing approximately 1 cm beyond the lesion margin.
   4. Cover the wet dressing with dry sterile gauze. Secure the dressing using hypoallergenic tape without compression.
   5. Leave the dressing in place for 4 h. Remove the dressing at the end of the scheduled treatment period.
   6. Clean the skin gently with sterile normal saline after dressing removal. Dry the area with sterile gauze.
   7. Repeat the treatment twice daily for 14 consecutive days. Maintain the same treatment schedule used in the experimental group.
   8. Record treatment completion, dressing tolerance, local skin reactions, and protocol deviations after each application. Use identical documentation procedures for both treatment groups.

5. Standard nursing care

1. Train the nursing staff
   1. Train all participating nurses before enrolling the first participant using the same written protocol and standardized demonstration. Review the phlebitis grading criteria, skin preparation, treatment coverage, dressing application and removal, 4-h retention requirement, safety monitoring, adverse-event reporting, and documentation procedures.
   2. Require each participating nurse to perform the study-related procedures under supervision before independently administering study treatment. Confirm procedural competency using the same predefined treatment checklist and correct any deviations before independent participant care is permitted.
2. Provide standard nursing care
   1. Inspect the peripheral intravenous catheter insertion site during each nursing shift. Assess pain, erythema, swelling, induration, burning sensation, and catheter patency using the standardized phlebitis grading criteria¹⁶.
   2. Monitor participants for worsening phlebitis, suspected extravasation, local infection, or other infusion-related complications. Report clinically significant deterioration promptly to the responsible investigator or treating physician.
   3. Instruct participants to protect the affected limb and avoid pressure, scratching, or unnecessary manipulation of the inflamed area. Advise participants to report worsening pain, swelling, redness, blistering, exudation, or other new symptoms immediately.
   4. Record phlebitis grade, nursing interventions, participant tolerance, and clinically significant findings during each scheduled assessment. Apply identical nursing procedures to both treatment groups throughout the study.

6. Outcome assessment and measurements

1. Assess all study outcomes at baseline (Day 0), Day 7, and Day 14. Complete each assessment before the morning topical application using standardized assessment forms.
2. Assess phlebitis severity
   1. Evaluate phlebitis severity using the predefined Grade 0–IV classification. Assess pain, erythema, swelling, burning sensation, induration, and exudation before assigning the phlebitis grade¹⁷.
   2. Determine whether participants have persistent Grade II or higher phlebitis on Day 14. Record phlebitis grades at each scheduled assessment.
3. Ask participants to rate pain using a 10-cm visual analog scale (VAS), where 0 indicates no pain, and 10 indicates the worst imaginable pain. Record the VAS score at each scheduled assessment.
4. Measure inflammatory biomarkers
   1. Collect fasting venous blood samples between 06:00 and 08:00 at baseline (Day 0) and Day 14 using the same sampling procedure at both time points. Label each specimen with the participant study identification code, collection time, and transport the samples promptly to the central laboratory.
   2. Allow the collected blood samples to clot at room temperature for approximately 30 min. Process all specimens within 2 h of collection. Centrifuge the samples at 1,500 × g for 10 min at 4 °C using a refrigerated benchtop centrifuge.
   3. Separate the serum immediately after centrifugation and inspect each specimen for hemolysis, lipemia, or other visible abnormalities that could interfere with analysis. Analyze the separated serum on the day of collection; do not subject samples to long-term frozen storage or repeated freeze–thaw cycles.
   4. Measure interleukin-6 (IL-6) using an electrochemiluminescence immunoassay according to the manufacturer's instructions. Perform a high-sensitivity C-reactive protein (hs-CRP) measurement using the validated assay routinely employed by the central laboratory.
   5. Use the same sampling, processing, and analytical procedures for baseline and Day 14 specimens. Perform instrument calibration and internal quality control in accordance with the manufacturer's instructions and the central laboratory's standard operating procedures. Record biomarker results only after the corresponding analytical run satisfies the predefined laboratory quality-control criteria.
5. Calculate the number of days from treatment initiation until pain decreases to a VAS score of ≤1 and redness and swelling remain absent or mild without progression for at least 24 h. Record the time to symptom resolution for each participant.
6. Evaluate Traditional Chinese Medicine (TCM) syndrome scores at each scheduled assessment using the predefined scoring criteria. Calculate the total score for each participant.
7. Calculate the efficacy index according to changes in the total TCM syndrome score. Classify treatment responses using the predefined efficacy categories and calculate the total effective rate.
8. Standardize outcome assessment
   1. Train both outcome evaluators before participant enrollment using the predefined Grade 0-IV phlebitis criteria and TCM syndrome scoring criteria. Review the definitions of pain, erythema, swelling, burning sensation, induration, and exudation to establish a consistent interpretation of each grading category.
   2. Ask the two trained evaluators to independently assess phlebitis grade and TCM syndrome score using identical standardized assessment forms. Whenever feasible, maintain the same evaluator pair across scheduled assessments. Keep the evaluators unaware of treatment allocation whenever possible.
   3. Resolve discrepant assessments by discussion and consensus. If consensus cannot be reached, request adjudication by a senior investigator who is not involved in treatment administration. Record the final adjudicated score in the case report form.

7. Data collection and quality control

1. Collect study data
   1. Collect baseline demographic and clinical information immediately after enrollment and before randomization. Record participant characteristics, tumor treatment status, phlebitis grade, VAS pain score, inflammatory biomarker levels, TCM syndrome score, and other relevant clinical variables using standardized case report forms.
   2. Complete follow-up assessments on Day 7 and Day 14 according to the study schedule. Record treatment completion, outcome measures, nursing observations, adverse events, and protocol deviations at each assessment.
2. Manage study data
   1. Enter all study data into the electronic database using independent double-data entry. Compare the two datasets and resolve discrepancies by reviewing the original source documents.
   2. Review completed case report forms for completeness and consistency before database entry. Correct identified errors promptly and document all modifications according to the study data-management procedures.
3. Maintain data quality
   1. Review study records regularly to identify missing, inconsistent, or implausible data. Verify questionable entries against the original case report forms and nursing records.
   2. Resolve all outstanding data queries before the database lock. Finalize the verified dataset before initiating the statistical analysis.

8. Safety monitoring

1. Monitor treatment safety
   1. Inspect the treatment area before each topical application and at each scheduled assessment. Evaluate erythema, swelling, blistering, dermatitis, exudation, pruritus, pain, and other local skin reactions.
   2. Document the onset, severity, duration, management, outcome, and relationship of each adverse event to the study intervention. Record all findings in the case report form.
2. Manage adverse events
   1. Classify adverse events as mild, moderate, or severe according to the predefined study criteria. Refer clinically significant events to an independent investigator for review.
   2. Stop the assigned topical treatment if severe irritation, suspected infection, allergic reaction, or other clinically significant intolerance develops. Provide appropriate medical management and document the reason for treatment discontinuation.
3. Reassess the treatment site after completion of the 14-day intervention. Document any persistent or delayed adverse reactions before study completion.

9. Statistical analysis

1. Prepare the study dataset
   1. Perform the primary efficacy analysis according to the intention-to-treat principle. Perform supportive analyses using the per-protocol population and evaluate safety in all participants who receive at least one topical treatment.
   2. Present continuous variables as mean ± standard deviation or median (interquartile range), as appropriate. Present categorical variables as frequencies and percentages.
2. Compare continuous variables using the independent-samples t-test or Mann–Whitney U test, as appropriate. Compare categorical variables using the chi-square test or Fisher's exact test.
3. Compare the proportion of participants with persistent grade II or higher phlebitis on Day 14 between treatment groups. Report effect estimates with 95% confidence intervals and corresponding P values.
4. Compare VAS pain scores, hs-CRP concentrations, IL-6 concentrations, TCM syndrome scores, and clinical efficacy between groups using appropriate parametric or nonparametric statistical tests. Analyze time-to-symptom resolution using Kaplan–Meier survival analysis with the log-rank test.
5. Perform additional analyses
   1. Adjust for clinically relevant baseline variables when appropriate using multivariable regression models¹⁸. Include prespecified variables such as baseline phlebitis grade, puncture site, and chemotherapy-drug irritability when applicable¹⁹. Report adjusted effect estimates with 95% confidence intervals.
   2. Compare the results of the intention-to-treat and per-protocol analyses to evaluate the robustness of the study findings.
6. Handle missing data and statistical significance
   1. Handle missing outcome data using the predefined statistical analysis plan. Perform sensitivity analyses when missing data are present.
   2. Perform all statistical analyses using SPSS Statistics version 26.0. Use two-sided statistical tests and consider P < 0.05 statistically significant.

Results

A total of 94 participants were randomized equally to the experimental and control groups (47 participants per group). Participant screening, randomization, follow-up, and analysis are summarized in Figure 1. The standardized intervention workflow, including topical treatment, standard nursing care, scheduled assessments, and safety monitoring, is illustrated in Figure 2. Baseline phlebitis grade, visual analog scale (VAS) pain score, high-sensitivity C-reactive...

Discussion

The principal methodological finding of this study is that the topical Huzhang Sanhuang formula can be reproducibly integrated into a standardized nursing pathway for chemotherapy-induced phlebitis²⁰. Successful implementation of the method is best demonstrated by concordant improvements in phlebitis severity, pain, symptom resolution, inflammatory biomarkers, treatment adherence, and safety. In the present study, participants receiving the Huzhang Sanhuang formula showed a lower incidence of pers...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
100-mesh stainless-steel filtration sieve	Hospital Pharmacy, The Second Affiliated Hospital of Nanjing University of Chinese Medicine	HZSH-FLT-100	Used to filter the decoction before concentration. Use the same 100-mesh specification for every preparation batch.
50% magnesium sulfate solution	Hospital Pharmacy, The Second Affiliated Hospital of Nanjing University of Chinese Medicine	HZSH-MGSO4-2025-01 (simulated in-house batch)	Control intervention. Prepare as a 50% w/v solution; soak sterile gauze, cover the visible lesion plus a 1-cm margin, retain for 4 h, and apply twice daily for 14 days.
Case report forms, intervention logs, and nursing checklists	Study Team, The Second Affiliated Hospital of Nanjing University of Chinese Medicine	HZSH-CIP-CRF-V1.0	Study-specific forms used to record screening, randomization, topical applications, nursing-bundle completion, adherence, protocol deviations, outcomes, and adverse events.
Chinese Pharmacopoeia	Chinese Pharmacopoeia Commission	2020 Edition, Part I	Reference standard for authentication and quality assessment of Polygonum cuspidatum, Rheum palmatum, Phellodendron amurense, and Scutellaria baicalensis.
Digital temperature measuring instrument	Testo SE & Co. KGaA	testo 925, 0563 0925	Used to monitor decoction temperature at approximately 95–100 °C and concentration temperature at 70–80 °C.
Electronic analytical balance	Mettler-Toledo International Inc.	ME204E	Used to weigh 100 g of each crude herbal drug and to verify the final concentrated paste mass. Readability: 0.1 mg.
High-sensitivity C-reactive protein assay and analyzer	Roche Diagnostics	Tina-quant Cardiac hs-CRP III, 09188240190; cobas c 702	Used to measure hs-CRP in fasting venous blood on Day 0 and Day 14. Use the same analyzer and reagent lot whenever possible.
Huzhang Sanhuang topical paste application measuring guide	Study Team, The Second Affiliated Hospital of Nanjing University of Chinese Medicine	HZSH-AG-01	Standardized guide for applying approximately 0.20 g/cm² as an approximately 2-mm-thick layer over the visible inflammatory area and 1 cm beyond the lesion margin.
Hypoallergenic paper surgical tape	3M Company	3M Micropore 1530-1, 2.5 cm × 9.1 m	Used to secure sterile gauze without compression during both experimental and control topical applications.
IBM SPSS Statistics	IBM Corporation	Version 26.0	Statistical software used for the prespecified analyses, including chi-square/Fisher exact tests, t tests, nonparametric tests, linear mixed models, Kaplan–Meier/log-rank analysis, Cox regression, and logistic regression.
Interleukin-6 assay and analyzer	Roche Diagnostics	Elecsys IL-6, 07027532190; cobas e 801	Used to measure IL-6 in fasting venous blood on Day 0 and Day 14. Use the same analyzer and reagent lot whenever possible.
Peripheral intravenous catheter, 22G	Becton, Dickinson and Company	BD Insyte Autoguard 22G × 1.00 in, 381423	Peripheral venous indwelling catheter used for chemotherapy administration; record insertion site, side of cannulation, and dwell time.
Peripheral intravenous catheter, 24G	Becton, Dickinson and Company	BD Insyte-N Autoguard 24G × 0.56 in, 381411	Peripheral venous indwelling catheter used for chemotherapy administration; record insertion site, side of cannulation, and dwell time.
Pharmacy refrigerator	Qingdao Haier Biomedical Co., Ltd.	HYC-310	Store the prepared Huzhang Sanhuang paste at 2–8 °C. Use each batch within 48 h and prepare a fresh batch every 2 days.
Phellodendron amurense bark (Huangbai)	Anhui Xiehecheng Pharmaceutical Decoction Pieces Co., Ltd.	Simulated lot HB20250115	Use 100 g per preparation batch. Authenticate according to the Chinese Pharmacopoeia; record supplier, lot number, authentication result, and certificate of analysis.
Polygonum cuspidatum root and rhizome (Huzhang)	Anhui Xiehecheng Pharmaceutical Decoction Pieces Co., Ltd.	Simulated lot HZ20250112	Use 100 g per preparation batch. Authenticate according to the Chinese Pharmacopoeia; record supplier, lot number, authentication result, and certificate of analysis.
Purified water system	Merck KGaA	Milli-Q IQ 7000	Supply 4,000 mL purified water for each 400-g herbal mixture, corresponding to a crude-drug-to-water ratio of 1:10 (w/v).
Rheum palmatum root and rhizome (Dahuang)	Anhui Xiehecheng Pharmaceutical Decoction Pieces Co., Ltd.	Simulated lot DH20250110	Use 100 g per preparation batch. Authenticate according to the Chinese Pharmacopoeia; record supplier, lot number, authentication result, and certificate of analysis.
Scutellaria baicalensis root (Huangqin)	Anhui Xiehecheng Pharmaceutical Decoction Pieces Co., Ltd.	Simulated lot HQ20250118	Use 100 g per preparation batch. Authenticate according to the Chinese Pharmacopoeia; record supplier, lot number, authentication result, and certificate of analysis.
Sealed wide-mouth storage bottle, 500 mL	Thermo Fisher Scientific	Nalgene HDPE bottle, 2114-0016	Used to store the concentrated Huzhang Sanhuang paste. Label each bottle with preparation date, batch number, storage temperature, and expiry time.
Sterile gauze swab, 10 cm × 10 cm, 8-ply	Winner Medical Co., Ltd.	Simulated item code WN-GS1010-8	Used to cover the Huzhang Sanhuang paste and to prepare the 50% magnesium sulfate wet dressing.
Sterile normal saline, 0.9% sodium chloride, 500 mL	Baxter Healthcare Corporation	VIAFLEX container, simulated reference 2B1323Q	Used to clean the affected skin before topical application and after dressing removal.
Temperature-controlled concentration apparatus	Shanghai Yarong Biochemical Instrument Factory	RE-52AA rotary evaporator	Used to concentrate the filtered decoction at 70–80 °C to approximately 1,000 g of homogeneous paste, equivalent to approximately 0.40 g crude drug/g paste.
Traditional Chinese medicine syndrome score form	Study Team, The Second Affiliated Hospital of Nanjing University of Chinese Medicine	HZSH-TCM-01	Standardized form covering local redness/erythema, swelling, burning sensation, pain/tenderness, and cord-like induration; each item is scored from 0 to 3.
Visual analog scale ruler	Study Team, The Second Affiliated Hospital of Nanjing University of Chinese Medicine	HZSH-VAS-01, 10-cm scale	Used to assess pain intensity from 0 (no pain) to 10 (worst imaginable pain) at Day 0, Day 7, and Day 14.
Water-based decoction apparatus	Beijing Donghuayuan Medical Equipment Co., Ltd.	YJX20/1+1D	Used to soak the 400-g herbal mixture in 4,000 mL purified water for 30 min and decoct at gentle boiling (approximately 95–100 °C) for 30 min.