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Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
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Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase

Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase

DOI:
10.3791/793-v

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10:20 min

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June 16, 2008

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1Department of Cell Biology and Virology,Heinrich-Pette-Institute for Experimental Virology and Immunology, University of Hamburg

Chapters

  • 00:07Title
  • 00:59What are limitations of today’s antiretroviral therapies
  • 01:41Excision of proviral DNA. Targeting LTR specific sequences.
  • 03:39Addressing the problem of multiple HIV isolates.
  • 04:35Generation of site-specific Tre recombinase via directed molecular evolution.
  • 05:49Delivery of Tre recombinase, HIV-1 proviral DNA excision, and elimination of HIV-1 viruses.
  • 06:39Open questions.
  • 08:21Towards a clinical application.

Summary

Automatic Translation

Automatic Translation

Current HIV-1 strategies act to suppress the viral life cycle but do not effectively eradicate infection. Here, we demonstrate that an engineered recombinase can efficiently excise integrated HIV-1 proviral DNA from the genome of infected cells.

Tags

HIV-1Proviral DNA ExcisionEvolved RecombinaseTreatment StrategiesResistant StrainsTre RecombinaseLoxLTRViral PseudotypesHeLa CellsVirus ProductionProof Of Principle

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