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Chapters
- 00:00Title
- 01:18Introduction
- 01:59Manganese Labeling of Human Embryonic Stem Cells
- 04:44Iron Labeling of Human Embryonic Stem Cells
- 06:34Performing Cellular Magnetic Resonance Imaging
- 08:41Analyzing MRI Results
- 10:18Conclusion
In this video, we are showing how to label human embryonic stem cells (hESC) with manganese chloride (MnCl2) which can enter cells via voltage-gated calcium channels when the cells are biologically active. Additionally, we show the use of MnCl2 as cellular MRI contrast agent to determine the in vitro viability of hESC.
