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JoVE Journal Biology
In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
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In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

Article DOI: 10.3791/827
August 3rd, 2008 •
1, 1
1Division of Cardiovascular Medicine, Stanford University

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August 3rd, 2008

In this video, we are showing how to label human embryonic stem cells (hESC) with manganese chloride (MnCl2) which can enter cells via voltage-gated calcium channels when the cells are biologically active. Additionally, we show the use of MnCl2 as cellular MRI contrast agent to determine the in vitro viability of hESC.

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