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Generation of Stable Transgenic C. elegans Using Microinjection

JoVE Journal
Biology

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JoVE Journal Biology

Generation of Stable Transgenic C. elegans Using Microinjection

Generation of Stable Transgenic C. elegans Using Microinjection

DOI:

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12:09 min

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August 15, 2008

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Laura A. Berkowitz, Adam L. Knight, Guy A. Caldwell, Kim A. Caldwell

¹Department of Biological Sciences,University of Alabama

Chapters

00:00Title
00:47Introduction
01:31Preparing for Microinjection
04:38Setting Up the Microinjection Microscope
07:16Injecting Expression Vector Constructs into C. elegans
10:31Screening for Transgenic C. elegans
11:41Conclusion

Summary

Automatic Translation

This video demonstrates the technique of microinjection into the gonad of C. elegans to create transgenic animals.

Tags

Transgenic C. Elegans Microinjection DNA Plasmid Concatamers Phenotypic Marker GFP Cellular Localization Studies Gene Expression Germline Early Embryo Experimental Paradigms Transgenic Worms Selection Maintenance

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