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Biology

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In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
 

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Article DOI: 10.3791/908
September 2nd, 2008

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Summary September 2nd, 2008

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

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