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Using Laser Tweezers For Manipulating Isolated Neurons In Vitro

JoVE Journal
Biology

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JoVE Journal Biology

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro

DOI:

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10:45 min

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September 11, 2008

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Robert Clarke, Jianfeng Wang, Ellen Townes-Anderson

¹Department of Neurology and Neuroscience, School of Medicine,University of Medicine and Dentistry of New Jersey – UMDNJ

Chapters

00:04Intro
01:00Fabrication of culture dishes with adhesive and non-adhesive surfaces
05:06Preparation of retinal cells
07:34Plating of cells on culture dishes
08:17Laser tweezer manipulation
10:07Observation of cell growth
10:45Acknowledgements

Summary

Automatic Translation

This video describes the manipulation of cultured neurons using laser tweezers in vitro.

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Laser Tweezers Isolated Neurons In Vitro Retinal Neurons Cell Processes Regenerating Cell Processes Retinal Cell Cultures Dissection Digestion Trituration Plating Laser Tweezers Dishes Sal-1 Antibodies Poly-HEMA Cell Repellant Cell Adhesive Laser-tweezers-controlled Micromanipulation Cell Selection Video Time-lapse

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