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Pressure-polishing Pipettes for Improved Patch-clamp Recording
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Pressure-polishing Pipettes for Improved Patch-clamp Recording

Pressure-polishing Pipettes for Improved Patch-clamp Recording

DOI:
10.3791/964-v

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05:12 min

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October 22, 2008

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, ,
1Department of Molecular and Cellular Physiology,Stanford University School of Medicine

Chapters

  • 00:00Title
  • 00:31Introduction
  • 01:00Pulling and Pressure Polishing Pipettes
  • 04:27Conclusion

Summary

Automatic Translation

Automatic Translation

This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.

Tags

Pressure-polishingPipettesPatch-clamp RecordingGlass PipettesShapingFabricatingNeuronal Cell BodiesGlass-blowingAir PressureHeatShape ModificationMicropipette PullerSoft GlassHard GlassPipette ResistanceTip Opening DiameterSoda Lime GlassBorosilicate GlassHigh-pressure ValveMicroforgeUltrasmall CellsSingle-channel RecordingBlunt ShapePerforated-patch Clamp Recording

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