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Biology

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Pressure-polishing Pipettes for Improved Patch-clamp Recording
 

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Article DOI: 10.3791/964
October 22nd, 2008

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Summary October 22nd, 2008

This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.

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