Name: Organotypic Slice Culture of E18 Rat Brains (Video) | JoVE Journal
Uploaded: 2007-07-11T00:00:00.000+00:00
Duration: 18 min 48 s
Description: Learn organotypic slice culture of E18 rat brains using vibratome sectioning and low melt agarose embedding. This technique enables long-term study of cortical development and neuronal migration in vitro.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

playerTrialDesktop

organotypic slice culture of e18 rat brains

Organotypic Slice Culture of E18 Rat Brains

Organotypic slice cultures from embryonic rodent brains are widely used to study brain development. While there are often advantages to an in-vivo system, organotypic slice cultures allow one to perform a number of manipulations that are not presently feasible in-vivo. To date, organtotypic embryonic brain slice cultures have been used to follow individual cells using time-lapse microscopy, manipulate the expression of genes in the ganglionic emanances (a region that is hard to target by in-utero electroporation), as well as for pharmacological studies. In this video protocol we demonstrate how to make organotypic slice cultures from rat embryonic day 18 embryos. The protocol involves dissecting the embryos, embedding them on ice in low melt agarose, slicing the embedded brains on the vibratome, and finally plating the slices onto filters in culture dishes. This protocol is also applicable in its present form to making organotypic slice cultures from different embryonic ages for both rats and mice. 

Organotypic slice cultures from embryonic rodent brains are widely used to study brain development. While there are often advantages to an in-vivo system, ...

My name is Laura Elias. I'm A neuroscience graduate student working in Arnold k Stein's lab. We're in the institute for re Regeneration medicine here at UCSF, and today I'm going to be showing you how to do organic typic slice culture of E 18 rat brains.So this procedure involves dissecting the brains out, embedding them in low melt agros, then slicing them on the viome and taking your corona sections, and then placing them in the Slice culture system. So there are a few important tools in reagents That we use for this slice culture procedure. You'll need a dissecting microscope to dissect out the brains.We also use this V like a VT 1000 S vibram, and that's a good system for cutting your slices. The glue can be important. We use this lock type for for glue that glues the brains very well because sometimes if you use the wrong type of glue, the brains will will come off when you're trying to cut them, and that can be problematic.We also use these milli cell filters that these are the filters that we plate are our slices on while they're in culture, and this is also an important Part of the process. All right, so before you start the Slice of culture process, there are a couple of o

organotypic-slice-culture-of-e18-rat-brains

Research

JoVE Journal

Biology

The organoptypic hippocampal slice culture model is an in vitro model used to examine neuronal injury in a variety of paradigms. In this article, we describe the methods for generating slice cultures and quantifying neuronal injury.

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions.  Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.

Organotypic Hippocampal Slice Cultures

This protocol describes an organotypic slice assay optimized for the postnatal brain and high-resolution time-lapse imaging of neuroblast migration in the rostral migratory stream.

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

This is a protocol to prepare and maintain a neocortical slice preparation in organotypic culture for the purpose of making electrical recordings from pyramidal neurons.

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

A method to generate organotypic slices from the E12.5 murine embryonic midbrain is described. The organotypic slice cultures can be used to observe the behavior of dopaminergic neurons or other ventral midbrain neurons.

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

This video demonstrates a method for preparing organotypic hippocampal slice cultures from the brain of a rat pup. It outlines the steps involved in the dissection of the hippocampus, slicing, and culture conditions required to promote cell viability and growth in the hippocampal slices, ultimately generating organotypic slice cultures.

Preparation of Organotypic Slice Cultures from a Rat Pup&#39;s Hippocampus

The video demonstrates a method for obtaining rhinal cortex-hippocampus slices from a rat pup's brain and culturing them in membranous inserts within a multi-well plate filled with media for further analysis.

Preparing Rhinal Cortex-Hippocampus Slices from a Rat Pup Brain

A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described.  This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Here we describe a technique for studying hippocampal postnatal neurogenesis using the organotypic slice culture technique. This method allows for in vitro manipulation of adult neurogenesis and allows for the direct application of pharmacological agents to the cultured hippocampus.

Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

We describe a protocol for cell-type specific expression of the genetically encoded FRET-based sensor ATeam1.03YEMK in organotypic slice cultures of the mouse forebrain. Furthermore, we show how to use this sensor for dynamic imaging of cellular ATP levels in neurons and astrocytes.

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Here, we describe the preparation of rhinal cortex-hippocampus organotypic slices. Under a gradual and controlled deprivation of serum, these slices depict evolving epileptic-like events and can be considered an ex vivo model of epileptogenesis. This system represents an excellent tool for monitoring the dynamics of spontaneous activity, as well as for assessing the progression of neuroinflammatory features throughout the course of epileptogenesis.

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

Learn organotypic slice culture of E18 rat brains using vibratome sectioning and low melt agarose embedding. This technique enables long-term study of cortical development and neuronal migration in vitro.

Organotypic Slice Culture of E18 Rat Brains

Explore More Videos