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Detectie van post-translationele modificaties op native Intact Nucleosomen door middel van ELISA
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Detectie van post-translationele modificaties op native Intact Nucleosomen door middel van ELISA

Article DOI: 10.3791/2593-v • 07:14 min • April 26th, 2011
April 26th, 2011 •
1, 2, 3, 2, 3
1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 2Department of Molecular and Cell Biology, University of Connecticut, 3Department of Pharmaceutical Sciences, University of Connecticut

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Nucleosoom ELISA (NU-ELISA) is een gevoelige en kwantitatieve methode om de wereldwijde patronen van post-translationele modificaties te detecteren in de voorbereiding van de inheemse, intacte nucleosomen. Deze wijzigingen zijn onder methylations, acetyleringen en fosforylaties op specifieke histon aminozuur residuen, en dus NU-ELISA geeft een globaal proteomics analyse van de totale chromatine modificatie staten van specifieke celtypen.

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Cellular Biology chromatine nucleosoom Epigenetica ELISA histon wijziging methylering acetylering
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