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In vitro Beredning av Active T. castaneum Telomeras

Article DOI: 10.3791/2799-v • 09:26 min • July 14th, 2011
July 14th, 2011 •
1, 1, 1
1Gene Expression and Regulation, The Wistar Institute, University of Pennsylvania

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Arbetet med att isolera den katalytiska subenheten av telomeras, TERT, i tillräckliga kvantiteter för strukturella studier, har haft en begränsad framgång i mer än ett decennium. Här presenterar vi metoder för isolering av den rekombinanta Tribolium castaneum TERT (

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Molekylärbiologi telomeras protein uttryck rening kromatografi RNA-isolering fälla
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