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A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo
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Bioengineering
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JoVE Journal Bioengineering
A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo

DOI:
10.3791/3581-v

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08:13 min

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January 04, 2012

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, , ,
1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Department of Biomedical Engineering, Materials Science Program, Laboratory for Optical and Computational Instrumentation,University of Wisconsin-Madison

Chapters

  • 00:05Title
  • 01:27Donor Cell Transfection
  • 03:33Intramyocardial Injection
  • 05:34Biophotonic Imaging
  • 07:24Biophotonic Imaging Sensitivity Result
  • 07:54Conclusion

Summary

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A method to track cell fusion in living organisms over time is described. The approach utilizes Cre-LoxP recombination to induce luciferase expression upon cell fusion. The luminescent signal generated can be detected in living organisms using biophotonic imaging systems with a sensitivity of detection of ˜1,000 cells in peripheral tissues.

Tags

Cre-Lox P RecombinationCell Fusion DetectionIn VivoMetazoansComplex OrgansSkeletal MuscleBonePlacentaTrophoblast CellsSyncytiotrophoblastMaternal-fetal BarrierCell FateStem Cell TransplantationSomatic CellsDifferentiationDevelopmentPathogenesis Of DiseaseBioluminescence

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