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Isolering og Primær kultur Rotte levercellerne
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Isolering og Primær kultur Rotte levercellerne

Article DOI: 10.3791/3917-v • 07:06 min • June 29th, 2012
June 29th, 2012 •
1, 2, 3, 1
1Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 2American University in Washington, D.C., 3Department of Internal Medicine, Saint Louis University School of Medicine

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Primære hepatocytter give et værdifuldt redskab til at vurdere biokemiske, molekylære og metaboliske funktioner i et fysiologisk relevant eksperimentelt system. Vi beskriver en pålidelig protokol for rotten in situ lever perfusion, som konsekvent genererer levedygtige hepatocytter op to1.0 × 10

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Cellular Biology fysiologi medicin primær cellekultur hepatiske celler hepatocyt rotte
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