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Journal / Bioengineering / Flexural Rigidity Measurements of Biopolymers…
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Bioengineering

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JoVE Journal Bioengineering
Flexural Rigidity Measurements of Biopolymers Using Gliding Assays
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Flexural Rigidity Measurements of Biopolymers Using Gliding Assays

Article DOI: 10.3791/50117-v • 07:55 min • November 9th, 2012
November 9th, 2012 •
1, 1, 1
1Department of Physics, Lawrence University

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A method to measure the persistence length or flexural rigidity of biopolymers is described. The method uses a kinesin-driven microtubule gliding assay to experimentally determine the persistence length of individual microtubules and is adaptable to actin-based gliding assays.

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Flexural Rigidity Measurements Biopolymers Gliding Assays Microtubules Cytoskeletal Polymers Cell Division Cell Mechanics Intracellular Transport Microtubule Persistence Length Stiffness Kinesin-driven Microtubule Gliding Assay Fluorescent Labeling Single Particle Tracking Nanometer-level Precision Automated Tracking Routine IDL
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