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JoVE Journal Biology
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
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Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Article DOI: 10.3791/51119-v • 07:28 min • February 27th, 2014
February 27th, 2014 •
1, 1, 1
1Huisken Lab, Max Planck Institute of Molecular Cell Biology and Genetics

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Summary
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The development of zebrafish can be followed over days with light sheet microscopy when embryos are embedded in optically clear polymer tubes with low-concentration agarose.

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Multilayer Mounting Long-term Light Sheet Microscopy Zebrafish Imaging Embryonic Development Photo-toxicity Bleaching Time-lapse Imaging Sample Mounting Strategy Low-concentration Agarose Optically Clear Tubes Real-time Developmental Biology
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