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Analys proteasomal Nedbrytning i ett cellfritt system i växter
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Analys proteasomal Nedbrytning i ett cellfritt system i växter

Article DOI: 10.3791/51293-v • 07:43 min • March 26th, 2014
March 26th, 2014 •
1, 1, 1
1Department of Biochemistry and Cell Biology, Stony Brook University, State University of New York

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Riktade nedbrytning protein utgör en viktig reglerande mekanism för cellernas funktion. Det sker via en konserverad ubiquitin-proteasom väg, som fäster polyubiquitin kedjor till målproteinet som sedan fungerar som molekylära "taggar" för 26S proteasom. Här beskriver vi en enkel och tillförlitlig cellfri analys för proteasomal nedbrytning av proteiner.

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Biokemi Ubiquitin / proteasomsystemet 26S proteasom proteinnedbrytning proteasomhämmaren Western blotting växt genetisk transformation
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