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JoVE Journal Developmental Biology
Effektiv Gene Transfer i Chick nethinder for primær cellekultur Studies
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Effektiv Gene Transfer i Chick nethinder for primær cellekultur Studies: An

Article DOI: 10.3791/52002-v • 08:59 min • November 2nd, 2015
November 2nd, 2015 •
1, 1, 1
1Wilmer Eye Institute, The Johns Hopkins University School of Medicine

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Embryonale chick retina cellekulturer udgør værdifulde værktøjer til undersøgelse af fotoreceptor biologi. Vi har udviklet en effektiv genoverførsel teknik baseret på ex ovo plasmid elektroporation af nethinder før kulturen. Denne teknik forøger transfektionseffektiviteten i øjeblikket tilgængelige protokoller, hvilket gør genmanipulation muligt for dette system.

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Developmental Biology retina fotoreceptor primær cellekultur elektroporering transfektion plasmid chick
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