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Journal / Biology / Isolating Intestinal Stem Cells from Adult…
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Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
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Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Article DOI: 10.3791/52223-v • 10:57 min • December 16th, 2014
December 16th, 2014 •
*1, *2, 1
1Institut für Biochemie und Molekulare Biologie, Universität Ulm, 2Institut für Immunologie, Universitätsklinikum Ulm
* These authors contributed equally

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Summary
Automatic Translation

Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. The method described here presents a simple and robust approach to enrich for and isolate Drosophila intestinal stem cells and the enteroblast progenitor cells by FACS at any time point during aging.

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Intestinal Stem Cells Adult Drosophila Midguts FACS Stem Cell Behavior Aging Tissue Homeostasis Molecular Mechanisms Intestinal Epithelium Molecular Markers Dissociation Of Midgut Tissue Living Cells Distinct Populations Esg-Gal4 UAS-GFP Fly Line Enteroblast Progenitor Cells
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