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Beeldvorming van de intracellulaire mensenhandel van APP met fotoactiveerbare GFP
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Beeldvorming van de intracellulaire mensenhandel van APP met fotoactiveerbare GFP

Article DOI: 10.3791/53153-v • 07:55 min • October 17th, 2015
October 17th, 2015 •
1, 1,2
1Department of Physiology and Pharmacology, Robarts Research Institute, Western University, 2Department of Clinical Neurological Sciences, Western University

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Hoewel het transport van celoppervlak-eiwitten relatief gemakkelijk bestudeerd visualiseren van de handel in intracellulaire eiwitten is veel moeilijker. Hier gebruiken we constructen waarin activeerbare GFP en tonen een werkwijze om nauwkeurig volgen amyloïd precursor eiwit van het Golgi apparaat naar stroomafwaartse compartimenten en volg de klaring.

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Cellular Biology Amyloid precursor protein foto-activeerbare GFP Golgi Lysosomen Intracellulaire trafficking ziekte van Alzheimer β-amyloid live-cell microscopie
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