Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / En Face Detection di ossido nitrico e superossido …
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
En Face Detection di ossido nitrico e superossido in strato endoteliale delle arterie Intact
Calcium Transient Measurements in hiPSC-CMs (Video) | JoVE
Journal (Biology)
Calcium Transient Measurements in hiPSC-CMs (Video) | JoVE
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
Journal (Biology)
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
 
Click here for the English version

En Face Detection di ossido nitrico e superossido in strato endoteliale delle arterie Intact

Article DOI: 10.3791/53718-v • 08:58 min • February 25th, 2016
February 25th, 2016 •
1,2, 1,2, 2,3, 1,2, 1,2
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg, 2Kidney Control of Homeostasis, Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

In questo articolo descriviamo un metodo relativamente semplice adattato utilizzando il colorante fluorescente diaminofluorescein-2 diacetato (DAF-2DA) e dihydroethidium (DHE) per en face rilevazione simultanea e la visualizzazione di ossido nitrico intracellulare (NO) e di anione superossido (O 2 .- ), rispettivamente, in appena isolato aortas intatte di un modello di obesità del mouse.

Tags

Biologia Molecolare Numero 108 arterie Microscopia confocale DHE DAF-2DA endotelio ossido nitrico anione superossido Biologia Vascolare
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter