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Journal / Biochemistry / 효소 활성 G4 Resolvase1의 정화를위한 G-quadruplex DNA 화성…
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Biochemistry

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JoVE Journal Biochemistry
효소 활성 G4 Resolvase1의 정화를위한 G-quadruplex DNA 화성 접근
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효소 활성 G4 Resolvase1의 정화를위한 G-quadruplex DNA 화성 접근

Article DOI: 10.3791/55496-v • 11:25 min • March 18th, 2017
March 18th, 2017 •
*1, *2, 3, 4, 1, 3
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
* These authors contributed equally

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G4 Resolvase1는 G4 결합 단백질에 대한 소감보고 된 친화 G-quadruplex (G4) 구조에 결합 HeLa 세포에서 G4-DNA 풀기 활동의 대부분을 나타냅니다. 우리는 특히 촉매 활성 재조합 G4R1를 정화 친 화성 및 G4-Resolvase1의 ATP 의존 풀기 활동을 마구를 채우는 새로운 프로토콜을 설명합니다.

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생화학 문제 (121) G4 Resolvase1, G-quadruplex ATP 의존적 용출 G-quadruplex 친 화성 단백질 정제 DNA 헬리 케이즈 RNA 헬리 카제
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