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Journal / Immunology and Infection / Fluorescence-based Neuraminidase Inhibition Assay …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
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Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Article DOI: 10.3791/55570-v • 09:31 min • April 15th, 2017
April 15th, 2017 •
1, 1
1WHO Collaborating Centre for Reference and Research on Influenza, Peter Doherty Institute for Infection and Immunity

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Summary
Automatic Translation

We describe the use of a phenotypic fluorescence-based neuraminidase inhibition assay to assess the susceptibility of influenza A and B viruses to the neuraminidase inhibitor class of antivirals.

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Fluorescence-based Neuraminidase Inhibition Assay Influenza Viruses Susceptibility Neuraminidase Inhibitor Antivirals Methodology Resistant Sensitive Principle Enzyme Activity Antiviral Susceptibility Testing Viral Titer Cell Cultures MDCK Cells Embryonate Chicken Eggs H1N1 H3N2 Subtypes U Bottom Plate Assay Buffer NP40 Multi-channel Pipette Dilutions Blank Well MUNANA Plate Sealer Incubation
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