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Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

Article DOI: 10.3791/55878-v • 09:20 min • August 10th, 2017
August 10th, 2017 •
*1, *1, 1, 1
1Section des Sciences Pharmaceutiques, Universités des Geneva–Lausanne (EPGL)
* These authors contributed equally

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Summary
Automatic Translation

The present method serves to identify isoform-specific inhibitors of histone deacetylases (HDAC) in HeLa cells by the UHPLC-MS analysis of multiple substrates. This is an antibody-free method developed to reflect HDAC1 and HDAC6 activity in the living cell environment, in contrast to single-isoform cell-free assays.

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HDAC1 HDAC6 UHPLC-MS HDAC Inhibitory Potential Selectivity Drug Discovery Living Cells Ultra High Performance Liquid Chromatography Mass Spectrometry Assay HDAC Isoforms Incubation Step Potency Selective Substrates Selective HDAC Inhibitors Endogenous Protein Acetylation Assays HeLa Cells Cell Lines Primary Cells Tumor Cells Clinical Specimens
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