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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Article DOI: 10.3791/55911-v • 09:32 min • September 10th, 2017
September 10th, 2017 •
1, 2, 1
1National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency

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A capillary-based immunoassay using a commercial platform to measure target proteins from total protein preparations is demonstrated. In addition, assay parameters of exposure time, protein concentration, and antibody dilution are optimized for a cell culture model system.

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Capillary Electrophoresis Immunoassay Automated Procedure Protein Targets Biological Sample Capillary Electrophoretic Immunoassays Western Blot Gels Transfer Devices Manual Washes Protein Required Rare Cell Types Limited Samples Three Hours Automated Systems Quantitative Reproducible Samples And Reagents Sample Buffer Dithiothreitol Fluorescent Master Mix Biotinylated Ladder Manufacturer's Product Insert Protein Samples BEAS-2B Cell Cultures
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