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Cancer Research

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JoVE Journal Cancer Research
Flow flowcytometri-baserede stof Screening System til identifikation af små molekyler, der fremmer Celledifferentiering glioblastom stamceller
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Flow flowcytometri-baserede stof Screening System til identifikation af små molekyler, der fremmer Celledifferentiering glioblastom stamceller

Article DOI: 10.3791/56176-v • 10:28 min • January 10th, 2018
January 10th, 2018 •
1, 1, 2, 1,3, 1
1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine

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En effektiv screening protokol præsenteres til identifikation af små molekyler, der fremmer astroglial differentiering i glioblastom stamceller (GSCs). Analysen er baseret på en stamcelle differentiering reporter hvorved udtryk for forbedrede normal god landbrugspraksis (eGFP) er drevet af den menneskelige GFAP promotor.

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Kræft biologi sag 131 stamceller gliom Astrocytic differentiering Lentivirus Reporter System stof skærmen differentiering terapi Acetyl cholin Calcium signalering
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