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JoVE Journal Developmental Biology
Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue
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Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue

Article DOI: 10.3791/56316-v • 08:05 min • September 26th, 2017
September 26th, 2017 •
1, 1, 1
1Departments of Anatomy and OB/GYN-RS, University of California, San Francisco

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We provide a protocol for imaging intracellular pH of an epithelial stem cell lineage in live Drosophila ovarian tissue. We describe methods to generate transgenic flies expressing a pH biosensor, mCherry::pHluorin, image the biosensor using quantitative fluorescence imaging, generate standard curves, and convert fluorescence intensity values to pH values.

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Intracellular PH Follicle Stem Cell Lineage Live Drosophila Ovarian Tissue Imaging Methods Stem Cell Biology Developmental Biology Intracellular PH Measurement Sulfate Decisions Mounting Chambers Vacuum Grease Cover Slip Dissection Buffer CO2 Gas Anesthesia Ovaries Forceps Ovarioles Muscle Sheath Separation
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