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Journal / Genetics / Immunostaining para modificações do DNA: análise…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
Immunostaining para modificações do DNA
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Immunostaining para modificações do DNA: análise computacional de imagens Confocal

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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Recém descoberto formas oxidadas de 5-metilcitosina (oxi-mCs), 5-Hidroximetilcitosina (5hmC), 5-formylcytosine (5-fC) e 5-carboxylcytosine (5caC) pode representar modificações de DNA distintas com funções únicas funcionais. Aqui, um fluxo de trabalho semi-quantitativa para visualização da distribuição espacial de oxi-mCs, perfis de intensidade de sinal e colocalization é descrito.

Tags

Genética edição 127 epigenética DNA oxi-mCs N6-methyladenine imuno-histoquímica Zen microscopia Confocal quantificação de sinal distribuição espacial
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