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Uma enzima de Split-TET2 projetado para Hydroxymethylation de DNA químico-inducible e remodelação epigenéticas
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Uma enzima de Split-TET2 projetado para Hydroxymethylation de DNA químico-inducible e remodelação epigenéticas

Article DOI: 10.3791/56858-v • 08:34 min • December 18th, 2017
December 18th, 2017 •
1, 2, 1
1Centre for Epigenetics and Disease Prevention, Department of Molecular and Cellular Medicine, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2Centre for Translational Cancer Research, Department of Medical Physiology, Institute of Biosciences and Technology, College of Medicine, Texas A&M University

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Protocolos detalhados para a introdução de uma enzima de divisão de engenharia-TET2 (cidra) em células de mamíferos para química inducible DNA hydroxymethylation e remodelação epigenéticas são apresentados.

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Química edição 130 metilação de DNA epigenética acessibilidade de cromatina demetilação do ADN biologia química TET2 5-Hidroximetilcitosina transcrição expressão gênica Epigenoma edição
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