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Journal / Genetics / En metode til at studere C924T polymorfi af…
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Genetics

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JoVE Journal Genetics
En metode til at studere C924T polymorfi af tromboxan A2 Receptor genet
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En metode til at studere C924T polymorfi af tromboxan A2 Receptor genet

Article DOI: 10.3791/57289-v • 07:00 min • April 1st, 2019
April 1st, 2019 •
1, 1, 2, 2, 2, 1, 3, 1, 2, 4, 1,2
1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences, University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 4CNR - Institute of Molecular Genetics, Section of Chieti

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I nuværende undersøgelse, vi beskriver en metode til at analysere C924T genotype. Protokollen består af tre faser: DNA-ekstraktion, forstærkning af Polymerasekædereaktionen (PCR) og analyse af begrænsning fragment længde polymorfisme (RFLP) på agarosegel.

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Genetik spørgsmålet 146 Molekylærbiologi Gen-ekspression Restriktionsenzymanalyse enkelt nukleotid polymorfisme genotypebestemmelse trombocytaggregation Atherothrombosis
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