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Journal / Immunology and Infection / Identifikation af virulens markører af…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifikation af virulens markører af Mycobacterium abscessus intracellulære replikering i fagocytter
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Identifikation af virulens markører af Mycobacterium abscessus intracellulære replikering i fagocytter

Article DOI: 10.3791/57766-v • 08:34 min • September 27th, 2018
September 27th, 2018 •
*1, *1, 1, 1, 2, 1, 1
12I, UVSQ, INSERM UMR1173, Université Paris-Saclay, 2Institut Pasteur, Unité de Pathogénomique Mycobactérienne
* These authors contributed equally

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Her præsenterer vi to protokoller for at studere phagocyt -Mycobacterium abscessus interaktioner: screening af en transposon mutant bibliotek for bakteriel intracellulære mangel og bestemmelse af bakteriel intracellulære transkriptom fra RNA sekventering. Begge tilgange giver indsigt i genomisk fordele og transkriptom tilpasninger forbedre intracellulære bakterier fitness.

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Immunologi og infektion sag 139 mikrobiologi amøbe Acanthamoeba castellanii Mycobacterium abscessus transposon bibliotek co kultur amøbe-mykobakterier RNA udvinding af intracellulære mykobakterier RNAseq.
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