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Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
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Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

Article DOI: 10.3791/58399-v • 09:54 min • November 4th, 2018
November 4th, 2018 •
1,2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 1,2,4
1Department of Surgery, Mayo Clinic, 2Midwest Fetal Care Center, Children's Hospitals and Clinics of Minnesota, 3Department of Surgery, Johns Hopkins, 4Pediatric Surgical Associates

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Summary
Automatic Translation

This protocol is intended to describe porcine hepatocyte isolation and ex vivo gene delivery to cure models of metabolic diseases via autologous cell transplantation. Although this particular model enjoys unique advantages that favor successful therapy, the application is a relevant foundation to address additional diseases and indications.

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Lentiviral Vector Gene Therapy Hepatocytes Ex Vivo Autologous Transplantation Inborn Errors Of Metabolism Liver Therapeutic Result Immunosuppression Hereditary Tyrosinemia Type 1 Platform Approach Visualizing Technique Perfusion Efficiency Viable Hepatocytes Surgical Procedure Cell Isolation Ex Vivo Transduction Animal Preparation OR Prep Port Site Entry
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