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Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
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Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling

Article DOI: 10.3791/58437-v • 05:47 min • February 12th, 2019
February 12th, 2019 •
1,2,3, 4,5,6, 4,5,6, 2,7, *1,2,3, *1,2,3,8
1Comprehensive Pneumology Center, Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München, Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration, Lund University, 5Wallenberg Center for Molecular Medicine, Lund University, 6Stem Cell Centre, Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado
* These authors contributed equally

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Summary
Automatic Translation

Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.

Tags

3D Lung Tissue Cultures Disease Modeling Living Human Tissue Natural 3D Structure Composition Laboratory Findings Potential Therapies Surgically Resected Lung Tissue Explanted Lung Tissue Deceased Patient Lung Tissue Samples Pulmonary Diseases Ex Vivo Pathomechanisms Cultivation Medium Culture Dish Metal Tray Tissue Paper Low Melting-point Agarose Peripheral Venous Catheter Bronchus Canula Forceps Bronchiole Wall Pulmonary Artery Surgical Clamp
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