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Immunology and Infection

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JoVE Journal Immunology and Infection
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Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

Article DOI: 10.3791/58949-v • 07:49 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1, 1
1McGovern Medical School, Department of Microbiology and Molecular Genetics, University of Texas Health Science Center at Houston

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Zebrafish (Danio rerio) are becoming a widely-used vertebrate animal model for microbial colonization and pathogenesis. This protocol describes the use of the protozoan Paramecium caudatum as a vehicle for food-borne infection in zebrafish larvae. P. caudatum readily internalizes bacteria and get taken up by larval zebrafish through natural preying behavior.

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Protozoan Paramecium Caudatum Vehicle For Food-borne Infections Zebrafish Larvae Microbiology Field Dynamics Of Colonization And Infection Gastrointestinal Tract Advantages Of The Technique Representative Of Food-borne Infections In Humans Reduced Tissue Damage In Fish Visual Demonstration Washing Steps Highly Motile Nature Of Paramecia Paramecium Culture E-coli MG1655 Culture Tissue Culture Flask E3 Medium Passaging Bacterial Half-life Within A Paramecium Count Optical Density Of 600 Paramecia Bacteria Co-culture Supernatant Microcentrifuge Tube Non-ionic Surfactant In PBS
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